1. General Announcements Exam 3Average score 66, high score 100, 10% curve applied Exam 3 RetakeDue Thursday 5 pm. Average score between in-class and retake will be recorded if higher than curved in-class exam. Quiz 3Average score 91 Review Session today 4:30 pm in Science 313

2. Glutathione (GSH) and Glutathione Disulfide (GSSG) Glutathione (GSH) Glutathione disulfide (GSSG) Is conversion of GSH to GSSG an oxidation or reduction reaction?

3. Glutathione (GSH) and Glutathione Disulfide (GSSG) Glutathione (GSH) Glutathione disulfide (GSSG) Oxidation Reduction

4. Glutathione is an Antioxidant Glutathione peroxidase (GSH peroxidase) Glutathione reductase (GSSG reductase) ROOH NADH Glutathione (GSH) Glutathione disulfide (GSSG)

5. Insulin Conversion of glucose to glycogen Oxytocin Labor, lactation Disulfide Bridges in Peptide Hormones

6. SAM Is Generated from the Methionine and ATP Methionine adenosyl transferase Driving force for the reaction is subsequent hydrolysis of triphosphate

7. S-Adenosylmethionine (SAM) Is A Biological Methylating Agent SAM-dependent methyl transferases O-, S-, and N-Methylation N-methylation: DNA and tRNA bases, amine side chains in proteins O-methylation: protein carboxylates, polysaccharide alcohols, neurotransmitter phenols S-methylation: Thiols S N 2 reaction

8. SAM at Work…

9. Methylation of Norepinephrine to Epinephrine by SAM and PNMT Phenylethanolamine- N-methyl transferase (PNMT) Adrenal cortex SAM Norepinephrine Epinephrine (adrenalin)

11. Introduction to Carbonyl Compounds Ketones Carboxylic acids AmidesEstersThioestersAnhydrides Acid chlorides Aldehydes LactonesLactams General reactivity towards nucleophilic attack