2. DELAYED-HYPERSENSITIVITY TESTING - DHT DELAYED-TYPE HYPERSENSITIVITY – DTH DELAYED CUTANEOUS HYPERSENSITIVITY - DCH

3. Delayed Cutaneous Hypersensitivity - DCH Process: This is a cell-mediated immunological response Purpose: - Diagnostic Testing 1. Existing infectious diseases: PPD tuberculin– Tuberculosis, Histoplasmin – Histoplasmosis and Coccidiooidin – Coccidiomycosis. 2. Immune system status: Mumps skin antigen, Candida, Fluid tetanus toxoid, Tuberculin PPD, and Trichophytin

4. Anergy and Anergy Panels Anergy is a defect in cell-mediated immunity. It is characterized by an impaired response or lack of a response to DH testing antigens. Anergy Panels: Three Antigen Panel: Candida, Mumps & Trichophyton Five Antigen Panel: Candida, Mumps, Trichophyton, PPD tuberculin & Tetanus Toxoid. Multitest – CMI: Candida, Trichophyton, Tetanus Toxoid Old Tuberculin, Diptheria Toxoid, Streptococcus, Proteus Antigen & Negative Control.

5. DH Testing Products CandidaCandida albicans (Candin) w/v (1:10 to 1:1000 w/v) Coccidioidin Coccidioides immitis (Biocox - Mycelial derivative ) 1:10 & 1:100 w/v ( Spherulin - Spherule derivative) 1:10 & 1:100 w/v Histoplasmin Histoplama capsulatum (Histoplasmin, Diluted - Filtrate from culture)1:100 w/v (Histolyn-CYL - Controlled Yeast Lysate) 1:100 v/v Mumps Skin Test Antigen Mumps virus MSTA40 CFU/mL (colony forming units) Tetanus Toxoid Clostridium tetani Fluid, for skin test & booster 10 Lf units / mL Adsorbed 8 Lf units / mL (Limit of flocculation) TrichophytinTrichophyton species1:100 to 1:1000 w/v Tuberculin, PPDMycobacterium tuberculosis (Purified Protein Derivative)1 tu (tuberculin unit ), 5 tu & 250 tu / 0.1 mL Administration: Inject 0.1 ID on the flexor surface of the forearm Interpretation: Read site for induration. Negative when No or < 5 mm, Positive ≥ 5 mm