1. What is happening with the culture of koi and tilapia in Hawaii? July 17, 2010 Haleakoakoa 101 Windward Community College

2. Workshop Agenda Introductions and expectations OPERATIONAL BIOSECURITY AND DIAGNOSTIC SURVEILLANCE, Clyde S. Tamaru Overview of Koi Herpes Virus by Dr. Allen Riggs Overview of Piscirickettsia-like organism (PLO) in tilapia Overview of the Shrimp Specific Pathogen Free (SPF) program: A potential model for other aquacultured species. COFFEE BREAK How does a species make it on to the list of species allowed to be imported into the islands. Vernon Nakamoto Goals and objectives to identify the various species and strains of tilapia currently in the islands.Harry Ako/Jinzen Yang Marketing tilapia in Hawaii? Is it a tough sell and what can we do about it? Todd Low Brainstorming Session: What are the next steps? Evaluation

3. Ground Rules Respect Others Listen to understand and avoid interrupting others Resist the temptation to put words into another persons mouth Be clear and concise Avoid side conversations while someone else has the floor Maintain an open and positive attitude Be open and non-defensive about your own ideas Everyone needs to participate\Put cell phones and pagers on the Joy setting

4. REGIONAL BIOSECURITY: OPERATIONAL BIOSECURITY AND DIAGNOSTIC SURVEILLANCE Charles Laidley, OPERATIONAL BIOSECURITY Amanda Lowrey and Clyde Tamaru, DIAGNOSTIC SURVEILLANCE

5. Stakeholder Input obtained via two workshops (e.g., Oahu and Hawaii

6. Diagnostic and Surveillance Objectives - Year 1: Objective 1: Establish a local diagnostic laboratory with PCR technology able to provide rapid turn around time. Objective 2: Hold workshop with Koi producers to solicit participation and input in establishing health status of Hawaii’s koi with regard to koi herpes virus disease (KHVD) Objective 3: Establish pro-active screening methods for KHVD Objective 4: Conduct initial survey of KHVD in farmed and feral koi populations statewide Objective 5: Produce technical handouts of resulting information. Information obtained is to also be included in the Operational Biosecurity handouts. Objective 6: Conduct follow up workshop to disseminate information and begin discussions on the challenges and opportunities of establishing a health certificate program for farmed koi.

7. Diagnostic and Surveillance Objectives - Year 2 Objective 1: Increase capacity of local diagnostic laboratory to include detection of Piscirickettsia-like organism (PLO) that infects tilapia. Objective 2: Conduct survey for Hawaii Tilapia Piscirickettsia-like organism (HTPLO) in farmed and feral tilapia populations statewide Objective 3: Extend survey to the whole CTSA region. Objective 4: Produce technical handouts of resulting information. Information obtained is to also be included in Operational Biosecurity handouts. Objective 5: Conduct follow up workshop to disseminate information and begin discussions on the challenges and opportunities of establishing a health certificate program for farmed tilapia.

8. Aquaculture: Percent of Total Sales State of Hawaii, 2008 45% 20% 24% 10% 1%

9. Tilapia is the fish used almost exclusively in aquaponics operations in Hawaii. Tolerates low DO levels (e.g., 0.2 ppm) Tolerates high total nitrate levels (>400 ppm) Tolerates high TAN levels (45 ppm) @ pH 6.0