1. BME 130 – Genomes Lecture 21 Genome replication

2. Figure 15.1 Genomes 3 (© Garland Science 2007) Genome replication Each strand of double helix serves as template for a new copy Problems?

3. Figure 15.2 Genomes 3 (© Garland Science 2007) Proposed models for DNA replication

4. Figure 15.3a Genomes 3 (© Garland Science 2007)

5. Figure 15.3b Genomes 3 (© Garland Science 2007)

6. The topological problem

8. Variations on the theme

9. Mitochondrial DNA replication

10. Rolling circle replication (lamba phage)

12. Replication origin in E. coli Found by testing sequence regions for ability to replicate associated DNA

13. Remain attached to DNA throughout cell cycle

14. Elongation

15. New bases are added to free 3’ OH groups in a template-directed fashion

16. Replication requires a free 3’ –OH and is going in two different directions!

18. Table 15.2 Genomes 3 (© Garland Science 2007)

20. Figure 15.15a Genomes 3 (© Garland Science 2007)

22. Figure 15.17 Genomes 3 (© Garland Science 2007) If the lagging strand loops back, the entire replication complex can move in a single direction (E. coli)

23. Linking adjacent Okazaki fragments

24. Figure 15.21 Genomes 3 (© Garland Science 2007) What if one side is faster?

25. Figure 15.22a Genomes 3 (© Garland Science 2007)

26. Figure 15.22b Genomes 3 (© Garland Science 2007)

27. Figure 15.23 Genomes 3 (© Garland Science 2007)

28. The end game

29. Figure 15.24a Genomes 3 (© Garland Science 2007)

30. Figure 15.24b Genomes 3 (© Garland Science 2007)

31. Figure 15.25 Genomes 3 (© Garland Science 2007)

32. Table 15.3 Genomes 3 (© Garland Science 2007)

33. Figure 15.26 Genomes 3 (© Garland Science 2007)

34. Figure 15.32 Genomes 3 (© Garland Science 2007)

35. Figure 15.33a Genomes 3 (© Garland Science 2007)

36. Figure 15.33b Genomes 3 (© Garland Science 2007)