1. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings PowerPoint ® Lecture Slide Presentation prepared by Christine L. Case Microbiology B.E Pruitt & Jane J. Stein AN INTRODUCTION EIGHTH EDITION TORTORA FUNKE CASE Chapter 6, part B Microbial Growth

2. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Deep-freezing: -50°to -95°C Lyophilization (freeze-drying): Frozen (-54° to -72°C) and dehydrated in a vacuum Preserving Bacteria Cultures

3. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Binary fission Budding Conidiospores (actinomycetes) Fragmentation of filaments Reproduction in Prokaryotes

4. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Binary Fission Figure 6.11

5. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Figure 6.12b

6. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings If 100 cells growing for 5 hours produced 1,720,320 cells:

7. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Figure 6.13

8. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Figure 6.14

9. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Plate Counts: Perform serial dilutions of a sample Direct Measurements of Microbial Growth Figure 6.15, top portion

10. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Inoculate Petri plates from serial dilutions Plate Count Figure 6.16

11. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings After incubation, count colonies on plates that have 25- 250 colonies (CFUs) Plate Count Figure 6.15

12. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Filtration Direct Measurements of Microbial Growth Figure 6.17a, b

13. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Multiple tube MPN test Count positive tubes and compare to statistical MPN table. Direct Measurements of Microbial Growth Figure 6.18b

14. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Direct Measurements of Microbial Growth Direct Microscopic Count

15. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Direct Measurements of Microbial Growth Figure 6.19

16. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Turbidity Estimating Bacterial Numbers by Indirect Methods Figure 620

17. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Estimating Bacterial Numbers by Indirect methods Metabolic activity Dry weight