2. Neuronal properties of the Dopaminergic neuron, H-cell Dopamine ple, Ddc, VMAT, DAT Neuropeptide receptor: NPFR1 Serotonin receptor: 5-HT1A Glutamate Receptor: Glu-RI H-cell

3. tup regulates dopamine biosynthetic and transport genes pdm2 nub tup SoxN D BH1 HGTX esg sim Pros Ddc zfh1 wor ple NPFR1 DAT Glu-RI 5-HT1A VMAT Elav CG31757 SNF4A 

4. tup expression begins late s11

5. Proteins involved in catacholamine synthesis TyrosineL-DOPADopamine TH (ple) VMAT Ddc Catsup GTP BH4 GTPCH (Pu) DAT Catsup

6. Ddc is down regulated in tup mutants

7. Ddc Ab staining Ddc Ddc/GFP

8. Ddc enhancer analysis Grainyhead binds element I Drifter binds Cf1

9. tup represses NPFR1 expression in abdominal segments

10. Mapping Antp midline expression Antp is expressed in mVUMs in T1

11. Gene regulatory networks in the H-cell pdm2 nub tup SoxN ac/sc D BH1 HGTX esg sim Pros Ddc zfh1 wor ple NPFR1 DAT Glu-RI 5-HT1A VMAT Elav CG31757 SNF4A 