1. Slide 88, elastic artery (aorta), (10x obj.) TI TM TA elastic fibers

2. Slide 36, elastic artery (aorta), trichrome (10x obj.) TI TM TA elastic fibers NOT vasa vasorum

3. Slide 36, elastic artery (aorta), trichrome (40x obj.) TM TA elastic fibers vasa vasorum

4. Slide 30, muscular artery (4x obj.)

5. Vessel Tunics A. Tunica Intima endothelium subendothelium connective tissue smooth muscle (longitudinal fibers) internal elastic lamina B. Tunica Media vascular smooth muscle external elastic lamina (circular fibers) C. Tunica Adventitia connective tissue (longitudinal) Slide 30, muscular artery (40x obj.)

6. Slide 92, muscular artery w/ intimal thickening (40x obj.) Vessel Tunics A. Tunica Intima endothelium subendothelium connective tissue smooth muscle (longitudinal fibers) internal elastic lamina B. Tunica Media vascular smooth muscle external elastic lamina (circular fibers) C. Tunica Adventitia connective tissue (longitudinal)

7. Slide 95, muscular artery, trichrome (4x obj.)

8. Slide 95, muscular artery, trichrome (40x obj.) tunica media internal elastic lamina elastic fibers tunica adventitia external elastic lamina

9. Slide 95, medium vein, H&E (4x obj.)

10. Slide 95, medium vein, H&E (10x obj.) Tunica media 3-15 layers of VSMCs no external elastic lamina Tunica adventitiaTunica adventitia thicker than tunica mediathicker than tunica media larger veins have longitudinal smooth muscle in adventitialarger veins have longitudinal smooth muscle in adventitia Tunica intima endothelium subendothelium IEL generally not evident

11. Slide 95, medium vein, trichrome (4x obj.) tunica adventitia tunica media

12. Slide 95, medium vein, trichrome (10x obj.) tunica adventitia tunica media elastic fibers Note: IEL sometimes evident w/ trichrome stain

13. Arterioles Tunica intima very thin subendothelial layer generally lacking distinct internal elastic lamina Tunica media 1-3 layers of VSMCs no external elastic lamina Tunica adventitia thin and ill-defined Venules Tunica intima endothelium pericytes Tunica media 1-2 sparse layers of VSMCs no external elastic lamina Tunica adventitia thicker than tunica media Slide 95, arteriole and venule, H&E (40x obj.)

14. Slide 95, arterioles and venules, trichrome (40x obj.) arteriole venule

15. Slide 95, arteriole and venule (?), trichrome (40x obj.) arteriole? venule? Remember, the change in vessel size is gradual, so you’ll find intermediate sizes. To make classification easy, focus on vessels that are clearly one type or another.

16. Slide 95, arteriole and venule (?), trichrome (40x obj.) arteriole? (sectioned obliquely) venule?

17. Slide 95, small vein & artery, H&E (40x obj.) vein artery

18. Slide 95, small vein & artery, trichrome (40x obj.) vein artery

19. Slide 30, lymphatic vessel (10x obj.) Note number of white blood cells in lumen valve

20. Slide 40, trachea (10x obj.) Look in this layer for arterioles and venules

21. Slide 40, trachea (40x obj.) venule arteriole

22. Slide 102, capillaries in myocardium (40x obj.)

23. Slide 98, H&E (orientation) epicardium ventricular myocardium papillary muscle A-V valve leaflet coronary vessels atrial myocardium Look under endocardium to find Purkinje fibers chorda tendinae

24. Slide 98, H&E, (20x obj.) endocardium Purkinje fibers in “subendocardium” (note perinuclear space and large fiber size) cardiac muscle myocardium

25. Slide 98, H&E (orientation) epicardium ventricular myocardium papillary muscle A-V valve leaflet coronary vessels atrial myocardium Look under endocardium to find Purkinje fibers chorda tendinae

26. Slide 98, trichrome (20x obj.) endocardium Purkinje fibers (note perinuclear space and large fiber size) cardiac muscle

27. Slide 98, trichrome (20x obj.) papillary muscle A-V valve leaflet chorda tendinae to atrium to ventricle Note that distribution of elastic fibers faces blood flow.

28. Slide 99, H&E (orientation) look here for A-V bundle of His ventricular myocardium atrial myocardium aortic valve leaflet A-V valve leaflet cardiac skeleton

29. Slide 99, H&E (4x obj.) A-V bundle of His ventricular myocardium cardiac skeleton

30. Slide 99, H&E (20x obj.) A-V bundle of His cardiac skeleton

31. Slide 99, trichrome (orientation) look here for A-V bundle of His ventricular myocardium atrial myocardium aortic valve leaflet A-V valve leaflet cardiac skeleton

32. Slide 99, trichrome (4x obj.) A-V bundle of His ventricular myocardium cardiac skeleton

33. Slide 99, trichrome (20x obj.) A-V bundle of His cardiac skeleton

34. Slide 100, H&E (orientation) ventricular myocardium atrial myocardium aortic valve leaflet

35. Slide 100, Weigert (orientation) ventricular myocardium atrial myocardium aortic valve leaflet

36. Slide 100, Weigert (4x obj) to ventricle to aorta Note that distribution of elastic fibers faces blood flow. aortic valve leaflet

37. Slide 100, Weigert (4x obj) aortic side of leaflet ventricular side of leaflet aortic valve leaflet Note that distribution of elastic fibers faces blood flow.

38. Slide 102, H&E (orientation) ventricular myocardium aortic valve leaflet epicardium coronary vessels atrial myocardium