1. Lab 1 Lecture 1 Laboratory Safety Guidelines

2. Objectives Provide simple guidelines that may be useful in the event of fire, medical emergency, chemical, or biological spill or personal contamination in the laboratory. Minimize the risk of injury or illness to laboratory workers by ensuring that they have the information, support and equipment needed to work safely in the laboratory.

3. GENERAL GUIDLINES Conduct yourself in a responsible manner all the time in the laboratory. When first entering a laboratory, do not touch any equipment, chemicals, or other materials in the laboratory until you are instructed to do so. Follow all written and verbal instructions carefully. If you do not understand a direction or part of a procedure, ASK YOUR SUPERVISOR BEFORE PROCEEDING WITH THE ACTIVITY.

4. GENERAL GUIDLINES At the beginning of each lab, you will be given a chance to ask questions. If your unsure of some procedure. Always pay attention to any instructions given at this time. Read all procedures thoroughly before starting the experiment. If you are unprepared, you will be unsure of yourself, waste time, and have a good chance of making a mistake that leads to a problem.

5. GENERAL GUIDLINES Never work in the laboratory without the presence of the supervisor. Labels and equipment instructions must be read carefully before use. Set up and use the equipment as directed by your supervisor. Carefully label everything: plates, solutions, etc. This is very important to the organization and success of your experiments, in addition to the safety of yourself and others. Labels should include your name, the date, and a brief description of what the plate, vial, etc.

6. GENERAL GUIDLINES Be aware of warnings on the labels of all chemicals. The bases, stains, and culture mediums used in microbiology are generally harmless. Be Familiar with all equipments and glass- ware in the area. Water bath, mixers, hotplate stirrer, and other mechanical equipments have user’s instructions. Try to keep inner walls of bottle stoppers or crocks from touching tops of desks or shelves where they might pick up dust or other chemicals. If stopper has a flat top, it may be rested upside down on the shelf or bench. If it has penny head top, the stopper may held between the fingers.

7. GENERAL GUIDLINES Keep hands away from face, eyes, mouth, and body while using chemicals or lab equipment. Do not ever use "mouth pipette." Discard all waste at the end of your lab bench. Maintain a lab space free of paper, text and other clutter. Only keep lab manuals notebooks and the materials you are working with on the bench: clutter can lead to spills and confusion.

8. GENERAL GUIDLINES Working areas should be kept clean and organize at all times. Always work in a well- ventilated area. Do not eat food, drink beverages, or chew gum in the laboratory. Foods and drinks, in any form must not be or stored in, any laboratory working area, or in any refrigerator in those areas. Do not use laboratory glassware as containers for food or beverages.

9. GENERAL GUIDLINES Mobile phones MUST BE OFF during lab time. It is distracting and you have to be attentive to what your doing. Wear a protective laboratory coats and gloves when working. Dress properly during a laboratory activity. Long hair must be tied back, and jewelry must be secured. Shoes must completely cover the foot, NO sandals allowed on lab days.

10. GENERAL GUIDLINES Contact lenses might not be worn in the laboratory, inform the supervisor if you have to wear them. Cosmetics (other than hand creams applied after washing) must not be applied in any laboratory. Wash your hands with soap and water after performing all experiments. Smoking is not permitted.

11. HANDLING GLASSWARE AND EQUIPMENT Never handle broken glass with your hands. Use a brush and dustpan to clean up broken glass. Place broken glass in the designated glass disposal container. Examine glassware before each use. Never use chipped, cracked, or dirty glassware. If you do not understand how to use a piece of equipment, ASK THE TEACHER FOR HELP! Do not immerse hot glassware in cold water. The glassware may shatter. When inserting a glass pipette into a Pipette pump, DO NOT PUSH ON THE TIP OF THE PIPETTE. The pipette might break and puncture your hand. Push the pipette in near the top, close to the Pipette pump. Cleaning the glassware after use.

12. HEATING SUBSTANCES Make sure that your hair, clothing, and hands are in a safe distance from the hot plate all times. Using hot plate is only allowed in the presence of the supervisor. Heated glassware remain very hot for a long time. They should be set aside in a designated place to cool, and picked up with caution. Use tongs or heat protective gloves if necessary. Never look into a container that is being heated. Do not place hot apparatus directly on the laboratory desk. Always use an insulated pad. Allow plenty of time for hot apparatus to cool before touching it.

13. First Aids For any emergency, including fire, explosions, accidents, and medical emergencies, inform your supervisors to call 998 or 997. Public Safety Personnel will respond, determine whether additional assistance is needed and alert others who can help.Public Safety –Any injury caused by burns, explosions, falls, chemical splash, or injury from sharp objects is to be immediately reported to supervisor (Doctors, Demonstrators and/or Technicians)-

14. First Aids –If you or your lab partner is hurt, immediately (and loudly) yell out the supervisor's name to get the supervisor's attention. Do not panic. –Know the locations and operating procedures of all safety equipment including: first aid kit(s), and fire extinguisher. Know where the fire alarm and the exits are located. –Know what to do if there is a fire during a laboratory period; containers must be closed, and any electrical equipment turned off. –Treatment requiring more elaborate supplies should be done at Health Center.

15. First Aid Kits First Aid kits is maintained by University departments and offices must: -Be kept in sanitary condition; -Be limited to simple household supplies such as band-aids and sterile gauze pads; and include the following personal protective equipment: At least one pair of large size examination or laboratory gloves

16. Take Care