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Visualization of genomic data Genome browsers
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How many have used a genome browser ? UCSC browser ? Ensembl browser ? Others ? survey
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Visualization of genomic data Genome browsers
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Genome browsers Visualization of a gene >sequence ATGAAGTTATGGGATGTCGTGGCTGTCTGCCTGGTGCTGCTCCACACCGC GTCCGCCTTCCCGCTGCCCGCCGGTAAGAGGCCTCCCGAGGCGCCCGCCG AAGACCGCTCCCTCGGCCGCCGCCGCGCGCCCTTCGCGCTGAGCAGTGAC TGTAAGAACCGTTCCCTCCCCGCGGGGGGGCCGCCGGCGGACCCCCTCGC ACCCCCACCCGCAGCCAGCCCCGCACGTACCCCAAGCCAGCCTGATGGCT GTGTGGCCTACCGACCCGTGGGCAAGGGGTGCGGGTGCTGAAGCCCCCAG GGGTGCCTGGCTGCCCACTGCTGCCCGCACGCCTGGCCTGAAAGTGACAC GCGCTGGTTTGCCCAGCACAGAGGGGATGGAATTTTTATGCTGCTCCTTT AGCATTCTGATGAACAAATATCCTCCCCACCAGCACCACCACCTCAGAAA Chr5123.004.678 123.404.678124.987.012125.345.112 Flat files / tab files
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Genome browsers Visualization of a gene (Ensembl)
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Genome browsers Visualization of a gene (UCSC) Exon Intron UTR
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Genome browsers
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UCSC genome browser http://genome.ucsc.edu/ Easy to use Often updates, but not as often as Ensembl upload of personal tracks Ensembl browser http://www.ensembl.org/index.html Less easy to use Maintained/updated by several people Gbrowser http://www.gmod.org/GBrowse Genome browsers
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UCSC genome browser Basic functionalities Finding a gene by name by sequence Gene structure Sequence orthologues Single Nucleotide Polymorphisms Gene Sorter - sort according to expression, homology... Custom tracks
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BLAT genome Browser http://genome.ucsc.edu//
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BLAT genome Browser Using a search term or position eg Chr1:10,234-11,567
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BLAT genome Browser http://genome.ucsc.edu/
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BLAT genome Browser Using a protein or DNA sequence
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BLAT Blast Like Alignment Tool BLAT (2002) Very fast searches (MySQL database) Handle introns in RNA/DNA alignments Data for more that 30 genomes (human, mouse, rat…) Exon Intron Exon Splice sites
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Blat genome Browser
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BLAT genome Browser ”Details” Correct splice site ?
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BLAT genome Browser ”Details” Donor site | Acceptor site exon.... G | GT...intron...AG | exon...
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Blat genome Browser
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BLAT genome Browser ”Browser” Base, Center & Zoom Known genes Predictions RNA EST Conservation Expression
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BLAT genome Browser Center & zoom
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Forward/reverse direction Selected number of tracks
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BLAT genome Browser Sequence Orthologs
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“klick”
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BLAT genome Browser Sequence Orthologs
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SNPs
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Chromosomal locus A locus is a physical location on a chromosome p the ‘short arm’ q the ‘long arm’ A locus range may describe a location of a gene 22q11.21-q11.23 22q12.2 Chromosome22 Armq Band12 Sub-band2
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Chromosomal locus Searching with gene name
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Chromosomal locus Searching with locus range
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Custom tracks Upload your personal data Share data with colleagues Data need to be related to a reference organism
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Custom tracks
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Exercise 1.Basic understanding of the graphics 2.Effect of Single Nucleotide Polymorphisms (SNPs) 3.Finding Orthologue genes 4.Identify chromosomal locus for a gene
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