1. Xray crystallography Lasse Mårtensson, IFM-Chemistry

2. Ribosome

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5. Diffraction pattern from a protein obtained by xray crystallography © 2006 Academic Press

6. Electrondensity map

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16. Requirements: Pure protein of high concentration 10-50 mg/ml AND… Protein crystals How to obtain protein crystals??

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22. Crystallization robot

23. Terms used in Xray crystallography Crystal system Spacegroup Unit cell Assymmetric unit Miller indices hkl Braggs law Ewald sphere

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42. Ewald sphere with a radius of 1/ All lattice points that touch the lattice obey Bragg´s law

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44. Refinement of the structure

45. Quality index R-factor:

46. Ramachandran plot