1. Liver
Rebecca Nardi

2. Blood Supply 75% of blood supply from gut (Venous Portal system)
25% from Hepatic Artery
Blood flows from the Portal Triad to the Central Vein via Sinusoids
Lab 19, Slide 26

3. The Liver – A 3-D View Lab 19, slide 25

4. Portal Triad (+lymph!)

5. Portal Triad (+lymph!)
Lab 19, Slide 28

6. Cords (or Plates) and Sinusoids
Plates of Hepatocytes
Blood-filled Sinusoids
Lab 19, Slide 30
Space of Disse

7. Space of Disse Microvilli
The Space of Disse is continuous with the lumen of the sinusoids due to spaces between the endothelial cells as well as in the basal lamina.
Endocytic Vacuole
This holds back cells, but not proteins. Proteins made in the liver can easily move into the blood.
Endothelium
Microvilli

8. Central Vein
Lab 19, Slide 29

9. 80% of the hepatic lymph drains into the thoracic duct.
Plasma that remains in the perisinusoidal space drains via lymphatics in the OPPOSITE direction as blood – i.e. from hepatocytes to the triad, and finally to the hilum.
80% of the hepatic lymph drains into the thoracic duct.

10. Three Lobules Classic Lobule Portal Lobule Liver Acinus SHAPE
Polygon (hexagon)
Triangle
Ellipse (or diamond)
CENTRAL VEIN
Central
3 Tips
2 Tips (triads at other tips)
Portal triads at corners
Follows bile drainage
Reflects blood supply

11. Classic Lobule

12. Portal Lobule

13. Liver acinus 3 2 1 2 3

14. Bile
Made by hepatocytes – it is secreted into canaliculi between cells
Hering canals connect canaliculi to bile ducts
Moves in opposite direction from blood – towards the portal triad
Nasty stuff – Canaliculi are bounded by tight junctions

15. Bile Canaliculi – Low Power
Phosphotungstic Acid & Hematoxylin stains the canaliculi.

16. Bile Canaliculi
Space of Disse
Tight Junctions
Canaliculus in Cross-section

17. Kupffer Cells Macrophages, derived from monocytes.
They form part of the lining of the sinusoid, but do not form junctions with endothelial cells.

18. It is carrying blood to the hepatocytes.
Portal Vein
What is at the pointer?
Is it carrying blood/bile to/from hepatocytes?
It is carrying blood to the hepatocytes.

19. Where is the highest O2 Content?
C – Zone 1 of the Acinus C A B