1. Al-Stricken Soil Leaching Test PGR-Stricken Soil Recovery Test LCH 1

2. Plastic tube 30cmX2.5cm A-0 A-1 A-2 Cotton Plastic wrap Tape LCH 2

3. A-3A-4 Fine Soil Filling H 2 O wetting Cotton topping Front water wetting. stop when the Front reach the bottom of the tube Soil Plastic wrap Tape LCH 3

4. A-5A-6 Application of Soil Amending agent: Biological or non biological (apply at 2g,4g,6g,8g,or 10g dosage per tube) Elute with H 2 O evenly until the water front reach the bottom of the tube. then seal the top end LCH 4

5. A-7 Lay flat of the tube, sow radish seed, then evaluate of seedling height after growing for 5 days ( 5X24 hrs ) with illumination LCH 5

6. A-8Photo-taken with clean background or mustard green velvet as background Top Bottom LCH 6

7. A.9 Height Plant 10 9 8 7 6 5 4 3 2 1 Bottom Top Dry Weight PH LCH 7

8. Statistical analysis of the data A-10 Figure Table LCH 8

9. B-0 preparation as A0-A6 30 cm diameter length 120 cm Bottom Top 100 cm 60 cm20 cm LCH 9

10. B-1 planting of evenly. Healthy grown seedlings diameter length 120 cm BottomTop 100 cm 60 cm20 cm 30 cm LCH 10

11. Terminate after 180 D B-2 Growth data recorded photograph taken statistical analysis conducted & data plotted diameter length 120 cm BottomTop 100 cm 60 cm20 cm 30 cm LCH 11

12. B-3 Bottom Top Plant height distance from top (cm) 906030 LCH 12

13. C-0 soil preparation as A0-A6 25 cm length 120 cm LCH 13

14. 40 cm Piston soil column soil column been (push out of the column by piston) of 40cm fraction are collected in 3 pots C-1 LCH 14

15. C-2 Bottom 80-120cm fraction Top 40cm fraction middle 40-80cm fraction LCH 15

16. C-3 fraction 80-120cm fraction 40cm fraction 40-80cm (a) planting of pineapple seedling (b) growth analysis at 180 days after planting (c) statistical analysis conducted and Figures/table plotted LCH 16

17. Targets 1.Qualitative assessment of soil amendment effect 2.Interaction of soil and amending agents. Leaching in soil or withhold by soil. 3. How far (deep) can the amending agent leached. 4. Dosage effect and its conversion for field soil amendment. LCH 17

18. Top ： Control Bottom ： PGR-Stricken Soil LCH 18

19. A.Control B.10 ppm leached C. 100ppm leached D. PGR-Stricken Soil LCH 19