2. The Centre for Structural Biology and Structural Chemistry What is the CSBSC? Why was it established? The Australian Synchrotron The Replacement Research Reactor

3. The Australian Synchrotron

4. The Replacement Research Reactor

5. Small Molecule Structure Determination in the CSBSC The School of Chemistry houses one of the world’s best small molecule crystal structure analysis facilities led by Dr Peter Turner: Two CCD area detector diffractometers Temperature Range 18-400 K

6. Small Molecule Structure Determination in the CSBSC

7. Dr Peter Turner also leads ScRAPS: small molecule structure determination using synchrotron radiation at APS, Chicago, Illinois This allows very small and/or poorly diffracting crystals to be analysed.

8. Powder Diffraction A/Professor Brendan Kennedy heads our powder diffraction facility. The CSBSC has strong links in powder diffraction with ANSTO and ANBF.

9. Protein Structure Determination in the CSBSC Macromolecular structure determination is based in the School of Molecular and Microbial Biosciences – headed by Dr Mitchell Guss

10. Structure of PPLO

11. The dimer interface Buried surface area per monomer is 8570 Å 2

12. NMR structure determination Not all proteins can be crystallised and then NMR is used to determine structures NMR also provides complementary information since it is derived from solution data

13. NMR structure determination

15. Charge Density Determination Structure determination is now so precise that we can determine electron densities and, locating orbitals and determining orbital occupancies The facilities in Sydney are amongst the very best in the world and will soon get better when we take delivery of a new state-of-the art system

16. Charge Density Determination

18. SRIXE, XANES, and XAFS The CSBSC has Australia’s largest concentration of expertise in SRIXE, XANES, and XAFS and leads the world in the application of these techniques to bioinorganic chemistry Metals under consideration include: V, Cr, Fe, Co, Ni, Cu, Zn, As, Mo, Pt

19. Elemental maps obtained from an A2780 cell treated with cis-[PtCl 4 (NH 3 ) 2 ]. K Cl Ca Cu ZnPt Determination of Pt distributions using SRIXE

20. Pt(II) Pt(IV) Monitoring the fate of Pt(IV) How can we determine where and when Pt(IV) complexes are reduced - XANES spectroscopy

21. MicroXANES of Pt obtained from A2780 ovarian cancer cells treated with Pt(IV)

22. Acknowledgments CSBSC Richard Christopherson (MMB) Carolyn Dillon (EMU/Chem) Mitchell Guss (MMB) Dai Hibbs (Chem) Brendan Kennedy (Chem) Cameron Kepert (Chem) Peter Lay (Chem) Joel Mackay (MMB) Simon Ringer (EMU) Rob Robinson (ANSTO) Siggi Schmid (Chem) Money Dean of Science PVC (CST) PVC (Research) VC (SDF)