Presentation on theme: "World Cornea Congress, San Diego, CA"— Presentation transcript:
1 World Cornea Congress, San Diego, CA Mini-Simple Limbal Epithelial Transplantation (Mini-SLET) Technique for the Treatment of PterygiumErick Hernandez-Bogantes1, MD, Alejandro Navas1, MD, MSc,Enrique O. Graue Hernandez1, MD, MSc, Arturo Ramirez-Miranda1, MD, Guillermo Amescua2, MD, Alejandro Lichtinger1, MD1Department of Cornea and Refractive Surgery. Instituto de Oftalmologia ‘Fundacion Conde de Valenciana’, Mexico D.F., Mexico2Department of Ophthalmology, Bascom Palmer Eye Institute, Miller School of Medicine, Miami, FL, USAWorld Cornea Congress, San Diego, CAApril, 2015
2 None of the authors have any financial interest to disclose
3 IntroductionThe most common surgical techniques for pterygium removal includes leaving bare sclera, using a conjunctival or conjunctival-limbal autograft, coverage with amniotic membrane (AM) or the use of adjuncts like mitomycin CRecurrence rates among these techniques varies widely with reports between 3.3% and 40.9%Advantages of using an AM are:Improvement of epithelial cell migration and differentiationInhibition of angiogenesisInhibition of cicatrizationThe possibility to cover a large area without the need of harvesting healthy conjunctiva
4 PurposeOur innovative technique describes the use of an AM graft to cover the bare sclera area combined with a small autologous simple limbal epithelial transplant (Mini-SLET) to provide stem cells at the limbal area.
5 Surgical TechniqueA, Nasal, temporal or bilateral pterygium are adequate candidates. B, Resection of pterygium with conventional techniques leaving bare sclera. C, Placement of amniotic membrane and resection of 2x2 mm of epithelial limbal stem cells. D, Slicing of epithelial limbal strip into 6-8 pieces. E, Alignment of small limbal transplants close to the limbal area over the amniotic membrane. F, Placement of a second amniotic membrane covering the pieces. G, Placement of a soft contact lens.
6 Posoperative day 1, notice the epithelial stem cells in place under the covering amniotic membrane
7 Demographic, Preoperative and Postoperative Outcomes of Patients that Underwent mini-SLET GenderAgeEyeClinical GradingSite of pterygiumRecurrenceComplications1Male34ODT2BilateralNone282Temporal3Female46OST1Nasal467T3557Pyogenic granuloma626770831949OU
8 Preoperative and Postoperative Clinical Photographs of 2 Eyes that Underwent mini-SLET A, preoperative photograph of a 47-year-old male carpenter (case 9) with a history of bilateral pterygium. B, 1 day after the surgery. C, After 1 week the amniotic membrane is fully attached to the scleral surface, there is no epithelial defect and the limbal epithelial pieces are visible and in place.. D, 1 month. E, 3 months. F, Preoperative photograph of a 26-year-old student (case 6) with bilateral pterygium, the mini-SLET was performed in the temporal lesion. G, 1 day after the surgery. H, 1 week. I, 1 month. J, 3 months.
9 DiscussionSangwan et al(4) described simple limbal epithelial transplantation as a technique for the treatment of stem cell deficiency. Inspired by this technique, we decided to combine the use of an AM graft which serves as an ideal substrate to support the growth of epithelial progenitor cells. We hypothesize that the addition of the stem cells contained in the mini-SLET pieces could improve the cosmetic outcomes and reduce recurrence rates.
10 DiscussionFor patients with primary pterygium the justification of additional expense of AM might not be justified, however, patients with limited amount of conjunctiva, the need of future surgeries or patients with previous multiple surgeries, the mini-SLET technique might be helpful.
11 ConclusionsWe believe that the mini-SLET procedure is reproducible and an easy to learn surgical technique.It requires much less tissue than conventional conjunctival autografts.While having no early signs of recurrence, the mini-SLET offers the advantages of epithelial stem cells, which in a long-term may reduce the rate of recurrence significantly.
12 ReferencesCano-Parra J, Diaz-Llopis M, Maldonado MJ, Vila E, Menezo JL. Prospective trial of intraoperative mitomycin C in the treatment of primary pterygium. The British journal of ophthalmology May;79(5):Demirok A, Simsek S, Cinal A, Yasar T. Intraoperative application of mitomycin C in the surgical treatment of pterygium. European journal of ophthalmology Jul-Sep;8(3):153-6.Ozer A, Yildirim N, Erol N, Yurdakul S. Long-term results of bare sclera, limbal-conjunctival autograft and amniotic membrane graft techniques in primary pterygium excisions. Ophthalmologica Journal international d'ophtalmologie International journal of ophthalmology Zeitschrift fur Augenheilkunde. 2009;223(4):Sangwan VS, Basu S, MacNeil S, Balasubramanian D. Simple limbal epithelial transplantation (SLET): a novel surgical technique for the treatment of unilateral limbal stem cell deficiency. The British journal of ophthalmology Jul;96(7):931-4.Amescua G, Atallah M, Nikpoor N, Galor A, Perez VL. Modified simple limbal epithelial transplantation using cryopreserved amniotic membrane for unilateral limbal stem cell deficiency. American journal of ophthalmology Sep;158(3):