1. HFT 405,488 488nm 405nm 488nm 405nm Only 405 and 488 are reflected All other wavelengths are transmitted 488nm 405nm This is the first beam splitter that directs the excitation light towards the sample. The sample then fluoresces at higher wavelengths, but also reflects back the excitation light back towards the beam splitter. The reflected excitation light reflects back towards the laser, but all other fluorescing wavelengths proceed towards the detectors. If there is no HFT that reflects all the desired wavelengths, use the NT80/20, which reflects 80% of all light and transmits the other 20%.

2. 560nm 505nm BP 505-560 (Band pass filter) Only wavelengths between 505 and 560 are transmitted All other wavelengths are reflected Band pass filters transmit only the wavelengths between the two cut off numbers. All other wavelengths are reflected away and not collected.

3. KP605 (short pass filter) 605nm Wavelengths shorter than the cut off are transmitted. Wavelengths longer than the cutoff are reflected Short pass filters only transmit wavelengths shorter than the cut off number. All other wavelengths are cast aside and not collected.

4. LP575 (long pass filter) 575nm Wavelengths longer than the cut off are transmitted. Wavelengths shorter than the cutoff are reflected Long pass filters only transmit wavelengths longer than the cut off number. All other wavelengths are cast aside and not collected.

5. 490 nm NFT 490 All wavelengths above 490 are transmitted All wavelengths below 490 are reflected These beam splitters act similarly to long pass filters, except they reflect and direct the lower wavelengths along other paths, towards other detectors.

6. Mirror Plate The mirror reflects all wavelengths The plate blocks all wavelengths.