1. Derrick R. J. Kolling March 16, 2010 The Chemistry of Photosynthetic Water Oxidation: From Fundamentals to Biofuels Kolling Laboratory Current Members Yasmine Zeid Ben Blodgett Cameron Buchman Courtney Nichols James Board Rebecca Mead Shaheed Elhamdani Evan Riley Matthew Thompson Former Members Chase Turner

2. The lab has three main projects: 1.Assembly of the oxygen-evolving complex 2.[Mn 2+ ] in compartments of phototrophs 3.Metabolomic studies of Chlorella vulgaris We are looking for students who: 1.Would like to work >3 semesters 2.Are interested in writing grants 3.Enjoy challenging projects

3. Psychrophile, 12°C Antarctic benthic mat Oscillatoria sp. Thermophile, 55°C PSII crystal structure Genome sequenced Thermosynechococcus elongatus www-dsv.cea.fr/var www-biol.paisley.ac.uk cc Project 1: OEC Photoassembly We are studying photoassembly in photosynthetic extremophiles that grow at very different temperatures.

4. Project 2: Compartmental [Mn 2+ ] We are working to determine the concentration of Mn 2+ in the different compartments of phototrophic prokaryotes and eukaryotes. This will help us to determine the mechanism of photoassembly. Chlamydomonas reinhardtii Cytoplasm Stroma Lumen Chloroplast Cytoplasm Lumen [Mn 2+ ]? Synechocystis sp. PCC 6803

5. Project 3: Metabolomics We are developing a strategy to determine the partial metabolome of the lipid generation pathways in Chlorella vulgaris. This organism and its metabolism are of interest, because its lipids can be used to make biodiesel.

6. Barber & Iwata (2004) Science A trip from a leaf to the oxygen-evolving complex. Ferreira et al. (2004) Science The oxygen- evolving complex!

7. How do we probe photosynthesis? O 2 production Fluorescence S-State Trapping EPR Spectroscopy