1. Theory and Practice: Agronomic Practices for sowing true seed and transplanted seedlings
Mohinder Singh Kadian
CIP-SWCA

2. What is TPS? TPS is a sexually produced propagule
TPS can be open pollinated or hybrid
Hybrid TPS is produced by crossing
Hybrid TPS is better in vigor, yield and tuber uniformity etc.

3. Steps for hybrid true seed production
Planting of hybridizing block
- Staggered planting of male parent
- Sodium vapor lamps of 100/250 watts per 100 sq.m.
2. Hybridization
- Thinning of female flower bunches
- Collection of flowers and extraction of pollen grains
(Buzzers and vibrators are developed for pollen extraction)
- Pollen can be stored under dry and low temperatures

4. 4. Harvesting of berries and seed extraction
3 Pollination
- Dipping stigma of female parent
( If possible select self male sterile female parent to reduce the labour cost required for emasculation)
4. Harvesting of berries and seed extraction
- Use of Juice extractor to make pulp
- Treatment of pulp with 10% HCL.for 20 minutes
- Fresh seeds treated with 0.05% Sodium Hypochlorite for 10 minutes to disinfect surface fungi or bacteria
- Drying of seed in shade
- Packing of seed at 4-5% moisture level

5. Breaking of Dormancy of true seed
Treat seed with 2000 ppm GA3 for 24 hours
Wash the true seeds with water
Dry true seed under shade
Check the germination of seed before sowing in bed
Store seed in tight containers or plastic bags at cool temperature.

6. Agro-techniques for potato production from TPS.
Steps followed
Substrate for Nursery Beds
(50% +50% subsoil or riber-bed sand)
Nursery Bed Preparation
(Raised beds-6-8 “) X

7. Producing TPS Seedling
TPS sowing (10cm or 25x4cm)
Shade (mulching)
Fertilizer Application(as recommended)

8. Producing Potatoes from Seedlings
Field preparation
Transplanting of Seedlings
Irrigation and earthing up
Dehauling and harvesting
Suitable Areas

9. Production of Seedling Tubers
Bed Preparation
Fertilizer Application
TPS Sowing
Foliar spray of urea