1. See using a flow cytometry assay expression of 3 goat poly clonal antibodies to purified viral proteins in both T and B cells but not in T cells from a normal donor.. We have shown expression of XMRV proteins but next wanted to ask if that reflected the presence of infectious and transmissable virus 3 goat polyclonal antibodies to purified viral proteins Rat α- MuLV p30 Gag mAb XMRV Protein Expression in Purified Activated T and B Lymphocytes from CFS Patients

2. Prostate cancer cell line Transmission of XMRV from Activated PBMCs of CFS Patients to the Human Prostate Cancer Cell Line LNCaP

3. Using a technique known as spinoculation we transmitted virus from plasma and confirmed infectious particles by TEM next slide 21 positive of 25 (84%) Cell Free Transmission of XMRV from CFS Patients Plasma to LNCap

4. Early and late buds type C morphology Mature extracellular viral particles with condensed centrally located nucleoid surrounded by an outer membrane separated by an electron lucent area Having detected the presence of XMRV viral proteins and particles in the PBMC of CFS patients, we next asked if we could detect an immune response to XMRV in CFS patients Infectious whole virus budding from the cell membrane Transmission Electron Micrograph of C-type Retrovirus Particles Transmitted from CFS patient T-Cells to LNCaP

5. BaF3-ER-SFFV Env BaF3-ER SFFV Env WB: αSFFV Env mAb BaF3-ERBaF3-ER-SFFV Env BaF3-ER BaF3-ER-SFFV Env Antibodies in CFS Patients Plasma to XMRV Env