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TIGR gene locicDNA (bp) Number of introns % homology (nucleotides) Protein Molecular weight (kDa) % homology (amino acids) KOME Accession number % homology.

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Presentation on theme: "TIGR gene locicDNA (bp) Number of introns % homology (nucleotides) Protein Molecular weight (kDa) % homology (amino acids) KOME Accession number % homology."— Presentation transcript:

1 TIGR gene locicDNA (bp) Number of introns % homology (nucleotides) Protein Molecular weight (kDa) % homology (amino acids) KOME Accession number % homology with Ubc9 S. cerevisiae LOC_Os10g39120843410018.04100AK12122163.1 LOC_Os03g03130981486.518.0593.1AK06216161.7 Supplementary data 1: Detailed characteristics of OsSce1 and OsSce2 proteins.

2 Supplementary data 2: Detailed characteristics of rice FKBP proteins. The protein sequences were retrieved from the TIGR database (www.tigr.org). The molecular weight and isoelectric point were determined using EditSeq module of DNAstar. Localization was predicted based on transit peptide sequence using TargetP 1.1 (http://www.cbs.dtu.dk/services/TargetP/) and PSORT (http://psort.nibb.ac.jp/) databases. NLS prediction was done at PredictNLS server (http://cubic.bioc.columbia.edu/predictNLS/). The protein sequences retrieved from the TIGR database were used for domain analysis at the Pfam database (http://www.sanger.ac.uk/Software/Pfam/search.shtml). The names proposed in the brackets are the calculated molecular weight of the proteins based on their cDNAs.www.tigr.orghttp://www.cbs.dtu.dk/services/TargetP/http://psort.nibb.ac.jp/http://cubic.bioc.columbia.edu/predictNLS/http://www.sanger.ac.uk/Software/Pfam/search.shtml Os04g28420 (OsFKBP65) 65.565.31Cytoplasm Os01g62610 (OsFKBP20.1) 20.15.98Nucleus Os01g68710 (OsFKBP16) 16.89.59Mitochondria Os02g28980 (OsFKBP74) 74.85.11Cytoplasm Os01g38180 (OsFKBP57-1) 57.35.42Cytoplasm Os01g38229 (OsFKBP47) 47.34.60Nucleus Os01g38359 (OsFKBP45) 45.65.17Cytoplasm Os03g25140 (OsFKBP70) 70.45.37Cytoplasm Os02g52290 (OsFKBP12) 12.087.96Nucleus Os02g51570 (OsFKBP23) 23.48.91Chloroplast Locus IDMolecular weight (kDa) Isoelectric point Localization Domain architecture Os02g07220 (OsFKBP22) 22.26.88Cytoplasm

3 Os09g12270 (OsFKBP44-2) 44.15.82Nucleus Os09g32526 (OsFKBP16) 16.25.12Extracellular FKBP TPR Signal peptide NLS Os11g05090 (OsFKBP42-1) 42.46.16Cytoplasm 42.25.67Cytoplasm Os12g05090 (OsFKBP42-2) Os09g01650 (OsFKBP60-2) 60.14.93Cytoplasm Os09g01670 (OsFKBP44-1) 44.35.18Mitochondria Locus IDMolecular weight (kDa) Isoelectric point LocalizationDomain architecture Os08g42850 (OsFKBP27) 27.18.23Chloroplast Os08g41390 (OsFKBP64) 64.15.06Cytoplasm Os07g09040 (OsFKBP60-1) 60.08.02Chloroplast Os05g38370 (OsFKBP20) 19.87.19Nucleus Os06g45340 (OsFKBP21) 21.99.27Chloroplast Os04g36890 (OsFKBP57-2) 57.175.88Nucleus

4 Supplementary data 3: A protein phylogenetic tree was created according to the neighbor-joining method clustering strategy in ClustalX program and analyzed using TREEVIEW 1.6.5 software. The proteins falling under the same group are shown in same color. Os01g62610 Os01g38359 Os01g38180 Os01g38229 Os02g28980 Os04g28420 Os08g41390 Os03g25140 Os11g05090 Os12g05090 Os02g07220 Os07g09040 Os06g45340 Os08g42850 Os09g12270 Os04g36890 Os09g01670 Os09g01650 At4g25340 At1g18170 At1g73655 At5g55220 At5g48570 At3g54010 At3g21640 At1g20810 At4g19830 At3g60370 At5g13410 At3g10060 Os02g51570 At4g39710 At5g05420 At5g64350 At3g25230 Os05g38370 Os09g32526 At3g25220 Os02g52290 At 5g48580 At 3g55520 Os01g68710 At5g45680 At2g43560 At2g12340

5 OsFKBP20 NP_191111 AAF18387 NP_1075779 AAH03447 AAH68678 NP_014264 Consensus OsFKBP20 NP_191111 AAF18387 NP_1075779 AAH03447 AAH68678 NP_014264 Consensus OsFKBP20 NP_191111 AAF18387 NP_1075779 AAH03447 AAH68678 NP_014264 Consensus OsFKBP20 NP_191111 AAF18387 NP_1075779 AAH03447 AAH68678 NP_014264 Consensus OsFKBP20 NP_191111 AAF18387 NP_1075779 AAH03447 AAH68678 NP_014264 Consensus Supplementary data 4: Alignment report of the OsFKBP20 protein with other eukaryotic FKBPs. NP_014264- Saccharomyces cerevisiae; NP_191111- Arabidopsis thaliana; AAF18387- Drosophila melanogaster; NP_1075779- Oryctolagus cuniculus; AAH03447- Mus musculus; AAH68678- Xenopus laevis.

6 OsFKBP20 NP_191111 AAF18387 NP_1075779 AAH03447 AAH68678 NP_014264 Consensus OsFKBP20 NP_191111 AAF18387 NP_1075779 AAH03447 AAH68678 NP_014264 Consensus OsFKBP20 NP_191111 AAF18387 NP_1075779 AAH03447 AAH68678 NP_014264 Consensus

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