1. PROTEIN Dept. of Medical Nutrition Medicine School Padjadjaran University

2. PROTEIN  A compound of C,H,O and N molecules that bind in a fashion way to form amino acids  Amino acids bind to each other to form protein  Amino acids bound --> peptide bound  Protein Nitrogen (PN)  Non Protein Nitrogen (NPN)

3. Classification of amino acid 1. Essential amino acid (EAA)  Could not synthesized by the body  Should get from outside the body (Exogen)  Adults need 8 essential amino acids which are : isoleusin, leusin, lysin, phenilalanin, methionin, threonin, tryptophan, valin  Children need histidin & arginin adjacent to the 8 essential amino acids mentioned above

4. Essential amino acids 1. Isoleucine8. Valin 2. Leucine9. Histidin 3. Lysin10.Arginin 4. Methionine 5. Phenylalanine 6. Threonine 7. Tryptophan

5. 2. Non essential amino acids (Non EAA)  Synthesized by the body from nitrogen and amino acid carbon chain  Non essential amino acids are important to the body but not essential from food (exogen)

6. Non essential amino acids 1. Glycine11. Glutamine 2. Glutamic acid12. Hydroxy glut acid 13. Hydroxy lysine 3. Arginine14. Hydroxy proline 4. Aspartic acid 15. Thyroxine 5. Proline16. Norleucine 6. Alanine17. Cystine 7. Serine18. Taurine 8. Tyrosine19. Carnitine 9. Cystein 10. Asparagine

7. Protein classified as follow : 1. Complete Protein - High biologic value (HBV) - Contain all EAA - Sufficient quantum of EAA - Meet the body needs - Able to support growth and to maintain body tissue E.g. : egg, meat, poultry, fish & milk

8. 2. Half complete protein : - Contain all of EAA - Insufficient quantum of EAA - Able to maintain adult health - Unable to support children growth E.g. : rice, wheat, tapioca

9. 3. Incomplete Protein : - Contain fewer EAA - Lesser EAA quantum - Inadequate to maintain health - Unable to support growth E.g. : gelatin, zein on corn

10. Role of Protein Provide amino acids for :  Growth and development  Maintain the body tissues  Produce energy  Protein synthesis

11. Protein food sources  Protein in the food should provide adequate quality and quantity that meet the body need.

12. Animal Protein  High biological value  Complete protein  Equivalent to body protein composition  Digestible and well absorb by the intestine

13. Plant Protein  Within the plants, protein covered by cellulose wall  Plant proteins have lower bioavailability

14. 1.Animal protein food sources  Milk (contain 1-4% of protein)  Egg (± 12%)  Fish (10-20%)  Meat(18-20%)  Chicken

15. 2. Plant protein food sources  Grain products ; rice, wheat (flour)  Nuts ; (15-25%)  green bean (± 22,2%)  soybean (35%)  peanut (25,3%)  bean  kacang bogor (10%)  kacang mete (21,2%)

16. 3. Plant protein food sources :  Tempe (10-18%)  Oncom (13%)  Tauco (10,4%)  Tofu (7-8%)  Emping Melinjo (12%)  Santan murni (4,2%)

17. The proteins synthesized in the body if there were complete and adequate amount of amino acids. The quality of protein rely on : availability of all EAA ; adequate amount (quantum) that meet the body need.

18. Protein quality assessment  Assessment of protein quality base on EAA composition.  Protein with complete EAA in adequate amount is called Protein Reference or Provisional Amino Acid Pattern (PAAP)

19. Amino acids pattern (FAO/WHO, 1973) EAA mg.AA/gr.nitrogen  Isoleusin250  Leusin440  Lisin340  Fenilalanin200  Tyrosin180  AA contain S 220  Metionin220  Threonin250  Triptofan60  Valin310 Source : Wohl & Godhart, 2nd Edition

20.  EAA in the food which have lower concentration (the lowest concentration) than PAAP ---> Limiting Amino Acid (LAA)  Percentage (%) of LAA ---> Chemical Score. Protein Chemical Score ---> EAA value in the food compared to EAA in PAAP

21. EAA composition and Chemical Score EAA Prot.Ref. Rice Fish Corn Soybean Isoleusin 250 290 317 228333 Leusin 440 654 474 535484 Lysin 340 234 549 268 66% 395 Fenilalanin 200 353 231 351 309 Tyrosin 180 171 159 88% 217 201 Methionin 220 113 178 117 81% 86 60% Threonin 240 268 283 268 247 Tryptophan 60 66 62 219 42% 86 Valin 310 399 327 324 328 First LAA tryptophan tryptophan methionin Second LAAtyrosin lysin tryptphan Third LAA - methionin -

22. How to increase protein quality? - Increase protein quality in the food - Increase the LAA concentration ---> SUPPLEMENTATION

23. Two fashions on supplementation 1. LAA Supplements - Add crystallized of pure AA - Expensive, ineffective E.g. : premix rice

24. 2. Supplementation by one or more food which contain various LAA E.g. : Rice have first LAA, lysin, mix with peanuts which have first LAA, methionin

25. Protein metabolism  Protein digested by proteolytic enzymes starting in the stomach ---> in duodenum, ileum and jejunum ---> form amino acids  AA absorbed through the intestine by active transport mechanism and assisted by carrier protein  AA ---> portal vein ---> liver  AA from the liver ---> blood ---> distributed to the cell organs

26.  Protein excretion ---> faeces ---> Undigested Dietary Protein and endogen protein  Healthy individual ---> protein does not excreted through urine, but the metabolite does Protein Metabolic Waste Product ---> Urinary Nitrogen : urea and non protein nitrogen (creatinin and uric acid )

27. Amino Acid Pool  There are some amino acids in the body that ready to use by any chance they needed as energy reserve ; e.g. : albumin and skeletal muscle cell.  Amino acids reserve mentioned above is called AMINO ACID POOL

28.  Tissue amino acids and Amino Acids Pool are in an equilibrium and dynamic state.

29.  Protein excretion higher than protein intake will perform negative nitrogen balance --> reduce body protein --> hypoalbuminemia or hypoproteinemia  Protein intake higher than protein excretion --> positive nitrogen balance. Excess protein intake will increase production of urea, creatinin and uric acid in the body

30. Protein RDA on various condition :  Children : 1,2-2,2 gr/kg BW/day  Adult: 0,8-1,0 gr/kg BW/day  Pregnancy: adult + 12 gr/day  Lactating first 6 month : adult + 16 gr/day  Lactating second 6 month : adult + 12 gr/day  Lactating > a year: adult + 11 gr/day

31. Diseases related to protein metabolism disorder 1. PEM (Protein-Energy Malnutrition) Protein deficiency always happen simultaneously along with energy deficiency. Protein deficiency could occur in adequate energy intake --> KWASHIORKOR

32. 2. HOMOCYSTEINURIA Inborn error of metabolism disease. Occur in cystathionin synthetase enzyme deficiency. Methionin metabolite accumulated and huge amount of homocystein. Children usually died at adolescent. 3. PHENYLKETONURIA Inherited, due to phenylalanin synthetase enzyme deficiency.

33. Citric acid  - Keto glutaric acid Succinic acid Oxaloacetic Arginine Histidine Hydroxyproline Proline Glutamic acid Valine Aspartic acid Cystine Cysteine Methionine Glycine Serine Threonine Alanine Acetyl CoA FAT CARBOHYDRATE Pyruvic acid Isoleucin Leucin Lysine Phenylalanine Tyrosine Acetoacetate KREB’S CYCLE