1. BENTHIC MACROINVERTEBRATE AND PERIPHYTON MONITORING IN THE SUWANNEE RIVER BASIN IN FLORIDA 1: OVERVIEW AND BIOGEOGRAPHY

2. Periphytic Algae Micro- to macroscopic algae growing on hard surfacesMicro- to macroscopic algae growing on hard surfaces Major source of autochthonous (“in-stream”) primary productionMajor source of autochthonous (“in-stream”) primary production Respond to natural and human-created changes in water qualityRespond to natural and human-created changes in water quality Integrate environmental effects over timeIntegrate environmental effects over time

3. Benthic Macroinvertebrates Invertebrates retained by 0.5 mm mesh sieveInvertebrates retained by 0.5 mm mesh sieve Ecologically important group of animals in all aquatic ecosystems in the drainageEcologically important group of animals in all aquatic ecosystems in the drainage Also respond to natural and human-created changes in water quality and habitatAlso respond to natural and human-created changes in water quality and habitat Integrate environmental effects over timeIntegrate environmental effects over time

4. Suwannee Basin Florida animal biodiversity

5. Suwannee River Drainage Benthic Community Sampling in Florida Fla. Fish & Wildl. Conservation Commission (resource assessments) Fla. Fish & Wildl. Conservation Commission (resource assessments) Fla. Dept. of Environmental Protection (monitoring, impact assessment, stream bioassessment program) Fla. Dept. of Environmental Protection (monitoring, impact assessment, stream bioassessment program) Suwannee River WMD (SWIM monitoring, basin assessments) Suwannee River WMD (SWIM monitoring, basin assessments) USGS Biol. Resources Division (sturgeon & seatrout food resources) USGS Biol. Resources Division (sturgeon & seatrout food resources) USEPA (NPDES impact studies) USEPA (NPDES impact studies) Private consultants (point source impact studies, NPDES monitoring) Private consultants (point source impact studies, NPDES monitoring) Citizen groups Citizen groups

6. Suwannee River Water Management District Benthic Monitoring Program began in 1989; currently continuingbegan in 1989; currently continuing quarterly samplingquarterly sampling multiple gear types:multiple gear types: –Hester-Dendy, Periph. & dip net (streams) –Petite ponar (streams and lakes) mainly streams (some lake studies)mainly streams (some lake studies) same organization doing samplingsame organization doing sampling same taxonomistssame taxonomists

7. BENTHIC SAMPLING GEAR SAMPLING GEAR Used in SRWMD Program D-frame dip net Hester-Dendy sampler Petite ponar grab Periphytometer

8. Suwannee River Water Management District Benthic Monitoring Program Sampling design ( sites and methods ):Sampling design ( sites and methods ): –SRWMD –FDEP –FWCC –USGS Field sampling: Environmental Services and Permitting, Alachua, FLField sampling: Environmental Services and Permitting, Alachua, FL Taxonomy: Dr. John Epler (invertebrates)Taxonomy: Dr. John Epler (invertebrates) Mr. Michael Hein (algae) Mr. Michael Hein (algae)

9. Water quality sites  Water quality sites Wq / biology sites  Wq / biology sites  Inactive biology sites SRWMD SUW010 SUW100 SUW130 SUW150 SUW240

10. Suwannee River taxa composition (1990-1999 Periphyton data) Cyanobacteria18910108 Chlorophyta4625162614 Bacillariophyceae7810911710880 Other taxa31000 TOTAL145144143144102 SUW010SUW100SUW130SUW150SUW240

11. Suwannee River taxa composition (1989-2000 Hest.-Dendy data) Mollusca616121823 Crustacea510678 Oligochaeta2028191825 Ephemeroptera1739292826 Odonata121071311 Chironomidae9996819194 Trichoptera3227242128 Plecoptera2101299 Coleoptera2830181919 Other taxa4238283253 TOTAL263304236256296 SUW010SUW100SUW130SUW150SUW240

12. Suwannee River taxa composition (1989-2000 Hest.-Dendy data) Mollusca616121823 Crustacea510678 Oligochaeta2028191825 Ephemeroptera1739292826 Odonata121071311 Chironomidae9996819194 Trichoptera3227242128 Plecoptera2101299 Coleoptera2830181919 Other taxa4238283253 TOTAL263304236256296 SUW010SUW100SUW130SUW150SUW240

13. Species new to science: (Chironomidae) Rheopelopia sp (??) Corynoneura (3 spp?) Cricotopus sp Parakiefferiella sp Zalutschia sp Orthocladiinae genus ‘E’ Cladotanytarsus sp Dicrotendipes sp Einfeldia sp Fissimentum sp Glyptotendipes sp Paratanytarsus sp Polypedilum sp Stempellina sp Stictochironomus sp Tanytarsus spp (several)

14. Species new to science: (Bacillariophyceae) Anorthoneis dulcis – the first freshwater representative in a marine genus

15. Range extensions: Ephemeroptera (mayflies) Siphloplecton sp. Ephoron sp. Plecoptera (stoneflies) Helopicus bogaloosa Chironomidae (non-biting midges) Polypedilum halterale

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