1. Specific Emerging Topics in Food Microbiology I Listeria monocytogenes in seafood I 27, May 2013-SS 1 Listeria monocytogenes in seafood Mansooreh Jami, Ph.D Student Institute of Food Science Department of Food Science and Technology BOKU-University of Natural Resources and Life Sciences A Presentation for Specific and Emerging Topics in Food Microbiology

2. Specific Emerging Topics in Food Microbiology I Listeria monocytogenes in seafood I 27, May 2013-SS 2 Six identified species L. monocytogenes, L. innocua, L. welshimeri, L. seeligeri, L. ivanovii, and L. grayi Primary human pathogen L. monocytogenes Gram (+), non-spore, motile, rod Aerobic and facultative anaerobic Psychotropic and halo-tolerant The Listeria genus

3. Specific Emerging Topics in Food Microbiology I Listeria monocytogenes in seafood I 27, May 2013-SS 3 Ubiquitous presence Widely distributed in the environment L. monocytogenes is found in relatively low number but under certain conditions facilitate higher number Soil & vegetation Fecal water Animal feed Foodborne pathogen that causes listeriosis Listeriosis is a serious infection caused by eating foods contaminated with the bacterium L.monocytogenes. Significance of L. monocytogenes

4. Specific Emerging Topics in Food Microbiology I Listeria monocytogenes in seafood I 27, May 2013-SS 4 This disease affects primarily pregnant women, newborn, and adults with weakened immune systems. Pregnant women Flu-like illness, fever, muscular pain, headache or asymptomatic Spontaneous abortion, stillbirth, septicemia, meningitis. Non-pregnant adults Mild gastroenteritis, septicemia, meningitis. Symptoms

5. Specific Emerging Topics in Food Microbiology I Listeria monocytogenes in seafood I 27, May 2013-SS 5 Foodborne illness caused by L. monocytogenes 14 serotypes of L. monocytogenes have been designated 1/2a, 1/2b, 1/2c, 3a, 3b, 3c, 4a,4b, 4bX, 4c, 4d, 5, 6a, 6b Serotype 1/2a, 1/2b, and 4b account for 96% of human infections in the United States Food-borne listeriosis

6. Specific Emerging Topics in Food Microbiology I Listeria monocytogenes in seafood I 27, May 2013-SS 6 Circulation of L. monocytogenes

7. Specific Emerging Topics in Food Microbiology I Listeria monocytogenes in seafood I 27, May 2013-SS 7 Temperature Growth range = -1 to 45°C Optimum = 30 to 37°C Psychrotrophic (refrigeration temperature; <5 °C) Mesophilic (room to body temperature; 18-38 °C) Temperatures < 0 moderately inactivate LM LM can survive freezing Factors affecting growth and survival

8. Specific Emerging Topics in Food Microbiology I Listeria monocytogenes in seafood I 27, May 2013-SS 8 Acidity Typical pH range is 5.0 to 9.6 Optimum =neutral conditions ~6.0 - 7.0 Growth at pH of <4.3 has not been demonstrated LM can tolerate lower pH when kept at near refrigeration temperatures (vs. room & body temperatures) Most strains of LM are unlikely to grow in food products with pH < 5.2 Factors affecting growth and survival

9. Specific Emerging Topics in Food Microbiology I Listeria monocytogenes in seafood I 27, May 2013-SS 9 Water activity Water activity (aw) LM grows well at aw of >0.95; can multiply at aw of 0.90 Some LM can survive at aw of <0.90 Salt concentration Growth at 10% Survival at 25.5% Factors affecting growth and survival

10. Specific Emerging Topics in Food Microbiology I Listeria monocytogenes in seafood I 27, May 2013-SS 10 Fresh seafood Raw fish Raw mollusc Raw crustacean Ready to eat seafood Cold smoked fish Hot smoked fish Gravad Semi processed seafood L. monocytogenes in seafood

11. Specific Emerging Topics in Food Microbiology I Listeria monocytogenes in seafood I 27, May 2013-SS 11 Listeria as indigenous pathogens in aquatic environment The prevalence of L. monocytogenes in raw fish is quite low, ranging from 0 to 1% (Autio et al., 1999) to 10% (Jemmi & Keusch, 1994). An overall prevalence 3% of L. monocytogenes was observed in European fish (Davies et al., 2001). Swetha et al., 2012; L. monocytogenes was isolated from 8 % fish samples. Different geographical area, Different type of products The problems can arise when handling, processing or storing conditions provide favourable conditions for the growth of the pathogenic bacteria. Fresh seafood products

12. Specific Emerging Topics in Food Microbiology I Listeria monocytogenes in seafood I 27, May 2013-SS 12 Cold smoked fish: A w (0.89 – 0.99), pH (3.1-7.2), Temperature (25-28 ºC) Hot smoked fish: A w (0.89 – 0.99), pH (4.1 – 8.4), Temperature (70-90 ºC) Gravad: Aw (0.97-0.99), pH (6.1-6.3) L. monocytogenes in lightly preserved seafood 15,000 samples 15,000 samples 1988-2007 1988-2007

13. Specific Emerging Topics in Food Microbiology I Listeria monocytogenes in seafood I 27, May 2013-SS 13 Seafood/lightly preserved seafood implicated in human listeriosis

14. Specific Emerging Topics in Food Microbiology I Listeria monocytogenes in seafood I 27, May 2013-SS 14 a n = number of units comprising the sample; c = number of sample units giving values over m or between m and M b m = M EU criteria for L. monocytogenes

15. Specific Emerging Topics in Food Microbiology I Listeria monocytogenes in seafood I 27, May 2013-SS 15 Conclusion The prevalence of L. monocytogenes generally increases along the food production chain, mainly due to cross- contamination in the production plants making this pathogen a major threat to the seafood industry. Attention must be paid to the design of food-processing equipment and to the effectiveness of the cleaning and disinfecting procedures in the production facilities, based on the known imperative preventive measures, such as Good Agricultural Practices (GAP) in the primary production, Good Hygiene and Manufacturing Practice (GMP) and also Hazard Analysis and Critical Control Points (HACCP).

16. Specific Emerging Topics in Food Microbiology I Listeria monocytogenes in seafood I 27, May 2013-SS 16 Thanks for Your Attention!

17. Specific Emerging Topics in Food Microbiology I Listeria monocytogenes in seafood I 27, May 2013-SS 17 Referencec : www.slideshare.com 17