1. HIV Latency: Molecular Mechanisms Eric Verdin, MD Associate Director and Senior Investigator

2. HIV Latency: Establishment vs. Maintenance

3. HIV Latency at the Molecular Level

5. (Vorinostat, Romidepsin)

6. HIV Latency: Establishment vs. Maintenance shRNA Screen for Cellular Genes that Control HIV Latency

7. shRNA Screen Experimental Plan

9. Non validated shRNAs 25 shRNA/gene 18,900 human genes 1000 shRNA controls 495,000 shRNAs total Duplicate Screen shRNA Screen Vector and Library Bassik MC, Kampmann M, et al Cell. 2013 Feb 14;152(4):909-22.

10. shRNA Screen Experimental Plan

12. Protein Complexes That Control HIV Latency Latency Promoting Latency Suppressing

13. TOR Signaling

14. TOR Signaling and CD4 T Cell Subsets

15. Treg Induction and HIV Latency

18. Treg Induction Is Associated with Increased HIV Latency

19. TOR Signaling in T cells

20. Inhibition of m-TOR Suppresses Reactivation From Latency

22. Title Box HDACi Prostratin

23. Title Box

25. V ERDIN L AB - G LADSTONE /UCSF Shweta Hakre Leonard Chavez Kotaro Shirakawa Vincenzo Calvanese Emilie Bativelli Matthew Dahabieh S ILICIANO L AB - J OHNS H OPKIN Nina Hosmane Robert Siliciano W EISSMAN L AB -UCSF Jonathan Weissman Martin Kampmann Michael Bassik K ROGAN L AB -UCSF Nevan Krogan Erik Verschueren Natalie Gulbahce G REENE L AB - G LADSTONE /UCSF Jonathan Chan Warner Greene

26. Title Box

27. Infection Trajectories Leading to Latent HIV

28. The p-TEFb Complex Controls HIV Latency Latency Promoting Latency Suppressing

29. The PRC2 Complex Controls HIV Latency Latency Promoting Latency Suppressing

30. The mTOR-TGFβ Pathways Control HIV Latency Latency Promoting Latency Suppressing