1. Electronic Sensor Technology WWW.ESTCAL.COM1 VaporPrint TM of Infectious Bacteria Cultures Salmonella typhimurium Strep pneumonia Shigella flexneri Salmonella enteritidis Hemophilus influenza Enterococcus faecalis E. Coli O157E. Coli Fresh Nutrient Staph aureus Pseudomonas aeruginosa Klebsiella pneumonia Candida albicans Cultures Prepared by: Department of Pathology and Laboratory Medicine, State Public Health Laboratory of Nevada

2. Electronic Sensor Technology WWW.ESTCAL.COM2 An Investigation of Infectious Bacteria With a GC/SAW Electronic Nose Water based cultures were inoculated with 12 infectious bacteria and allowed to incubate at 37 o C for 24 hours before analyzing headspace vapors with GC/SAW Electronic nose. Two of the twelve cultures did not develop and contained no bacteria. Headspace vapor of cultures without bacteria displayed two primary nutrient analyte peaks and three small analyte peaks located midway between the two nutrient peaks. Bacteria growth dynamically interacted with nutrient compounds as well as created new analyte vapors specific to the bacteria itself. The concentrations of new analytes together with modified nutrient vapors created VaporPrints TM images specific to bacteria type. E.Coli and E.Coli )157 produced nearly identical VaporPrintsTM, however, one analyte was found to be specific to only O157. In general each bacteria type could be recognized by its unique VaporPrint TM image.

3. Electronic Sensor Technology WWW.ESTCAL.COM3 Strep pneumonia Nutrient Broth Hemophilus influenza Nutrient Broth No Growth Confirmed for Hemophilus influenza and Strep pneumonia Culture Bottles

4. Electronic Sensor Technology WWW.ESTCAL.COM4 Candida albicans Nutrient Broth

5. Electronic Sensor Technology WWW.ESTCAL.COM5 Shigella flexneri Nutrient Broth

6. Electronic Sensor Technology WWW.ESTCAL.COM6 Staphylococcus aureus Staph aureus Nutrient Broth

7. Electronic Sensor Technology WWW.ESTCAL.COM7 Pseudomonas aeruginosa Nutrient Broth

8. Electronic Sensor Technology WWW.ESTCAL.COM8 Salmonella enteritidis Nutrient Broth

9. Electronic Sensor Technology WWW.ESTCAL.COM9 Enterococcus faecalis Nutrient Broth

10. Electronic Sensor Technology WWW.ESTCAL.COM10 Klebsiella pneumonia Nutrient Broth

11. Electronic Sensor Technology WWW.ESTCAL.COM11 Escherichia coli O157 E. coli O157 Nutrient Broth

12. Electronic Sensor Technology WWW.ESTCAL.COM12 E. coli (benign) E. Coli (benign) Nutrient Broth

13. Electronic Sensor Technology WWW.ESTCAL.COM13 Comparison of E. coli E. coli O157 E. coli (benign) Specific to O157 E. Coli

14. Electronic Sensor Technology WWW.ESTCAL.COM14 Salmonella typhimurium Nutrient Broth