1. Histology Laboratories Molecules to Systems 2003 Compiled by James D. Jamieson, MD/PhD Thomas L. Lentz, MD No part of this image collection may be distributed outside of the Yale University Intranet.

2. Alberts, B. et al. Molecular Biology of the Cell. 4th Edition, Garland Science, New York, 2002. Gartner, L. P. and Hiatt, J. L. Color Atlas of Histology, Williams & Wilkins, Baltimore, 1994. Kerr, J. B. Atlas of Functional Histology. Mosby, London, 1999. Kessel, R. G. and Kardon, R. H. Tissues and Organs: a text-atlas of scanning electron microscopy. W. H. Freeman, San Francisco, 1979. Lentz, T. L. Cell Fine Structure. W. B. Saunders, Philadelphia, 1971. Lodish, H. et al. Molecular Cell Biology. W. H. Freeman, New York, 2000. Mizoguti, H. Color Slide Atlas of Histology. Nihon Shashin Shinbunsha, Tokyo. Young, B. and Heath, J. W. Wheater’s Functional Histology. Churchill Livingstone, Edinburgh, 2000. Micrographs taken by George Palade, Marilyn Farquhar, James D. Jamieson, Nicolai Simionescu, Maya Simionescu, David Castle, Thomas L. Lentz. Web Resources http://info.med.yale.edu/webpath/webpath.htm Cushing Library Educational Software/Cell Biology/Several Histology Resources Acknowledgements Sources of Micrographs, Diagrams and Figures

3. Epithelium Laboratory

4. Classification of epithelia - By layers Simple: single layer of cells. Stratified: two or more layers of cells; only the first layer attached to basement membrane (e.g, skin). Pseudostratifed: nuclei appear at different levels; all attached to basement membrane but not all reach cell surface (e.g., bladder). - By shape Squamous: flattened, plate-like (e.g, endothelia, mesothelia). Cuboidal: as tall as they are wide (duct linings). Columnar: taller than wider. Found in epithelia specialized for absorption, secretion; surface specializations for motility.

5. Simple Epithelia

7. Endothelial cell nuclei

8. Mesothelial cells

10. Kidney Collecting Ducts: XS

11. Kidney Collecting Ducts; LS

13. Intestinal epithelium with Brush border = microvilli

14. Pseudostratified Epithelia

16. Respiratory Epithelum with Cilia

17. Stratified Epithelia

19. Non Keratinized Stratified Squamous Epithelium. (vagina or esophagus)

21. Keratinized Stratified Squamous Epithelium Stratum germinativum (basale) Str. spinosum Str. granulosum Str. corneum Dermal ridge Melanocytes

22. Transitional epithelium

24. Relaxed Urinary Bladder

25. Gland Classification & Secretion Modes

28. Surface Specializations 1 Microvilli: LM: brush border as on intestinal epithelial cells. Reflects actin cores of microvilli seen by EM. EM: protrusions of apical plasma membrane from epithelial cells with an actin core. Vastly increases surface area for digestion and absorption.

29. Intestinal epithelium with brush border = microvilli Brush border Terminal bars (Junctional complexes) Terminal web

30. Microvilli LS; EM

31. Microvilli XS; EM

32. Surface Specializations 2 Cilia and flagella: LM: typical of apical surface of pseudostratified ciliated columnar epithelium of respiratory tract. Involved in moving goobers up the airway and in moving fertilized ovum down oviduct. EM: core of 9 + 2 microtubules responsible for whipping action of cilia. Flagella have same structure as cilia but are found singly on cells as in sperm.

33. Cilia

34. Cillia LS Garbage in airway Cillia XS Respiratory Epithelium ; EM

35. Cillia XS; EM