1. The Nucleus Text and image sources are included using the notes function of the.ppt file

2. Interphase Nuclear Dynamics DIC Brightfield Euchromatin Time Lapse Video

3. EM thin section view

4. Chromatin organization early replication -> late replication chromosomal organization one cell cycle later lamin B and late replicated DNA lamin B and early replicated DNA

5. Packing it all in 146 bp; 6x condensation 40x condensation

6. The Histone Code

7. Anchoring the genome LAPs (Lamin associated proteins) anchor the lamina to the inner envelope

8. Freeze etch view of the lamina

9. The nuclear matrix

10. Diversity of nuclear shapes

11. Eosinophil nuclei are constricted

12. Chromosomal domains

13. Nuclear compartments

14. Promyelocytic leukemia (PML) nuclear bodies Video

15. Total RNA production (fluorouridine pulse)

16. Nucleolar Substructure Mammalian cell Yeast cell

17. Polymerase I transcribes rDNA in nucleolar fibrillar centers 500 nm

18. Gene Expression Factories Animated reconstruction Euchromatin (acH3), DNA (DAPI), Spliceosomal protein SC-35

19. Spliceosomes contain poly-A RNA

20. Nuclear speckle dynamics Time lapse sequences show SC-35 protein over 30 min periods 3 Video Sequences

21. Nuclear mRNA localizations Collagen A1 mRNA Epstein-Barr Viral mRNAs mRNA (red) Gene (green)

22. Nuclear traffic

23. Visualizing Nuclear Pores Negative Stain Thin Section Freeze Fracture CryoEM Tomographic Reconstruction

24. Annulate lamellae store pores 400 nm

25. Nuclear pores In vitro regulation by Ca++

26. Transport of nucleoplasmin- coated gold particles

27. It takes two (or three) to tango Digitonin-permeabilized cells incubated with NLS*BSA*TRITC -cytosol +cytosol +cytosol, GTP

28. NLS*BSA + cytosolic factors identifies nucleoporins on a Western-style blot Nup358 Nup214 Nup153 Nup 98 Nup 62

29. Nuclear Transport Ran*GDP Ran*GTP Ran*GDP Ran*GTP Cytoplasmic compartmentNuclear compartment Ran-GEFRan-GAP Exportin NES Exportin NES NLS Importin NLS Importin

30. FG affinity transport

31. Follow the FG-repeats Follow the FG-repeats Follow, follow, follow, follow Animation

32. The nucleus - not just a place to find DNA anymore