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Effects of Autologous Platelet-Rich Plasma on Cell Viability and Collagen Synthesis in Injured Human Anterior Cruciate Ligament by Louay Fallouh, Koichi.

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Presentation on theme: "Effects of Autologous Platelet-Rich Plasma on Cell Viability and Collagen Synthesis in Injured Human Anterior Cruciate Ligament by Louay Fallouh, Koichi."— Presentation transcript:

1 Effects of Autologous Platelet-Rich Plasma on Cell Viability and Collagen Synthesis in Injured Human Anterior Cruciate Ligament by Louay Fallouh, Koichi Nakagawa, Takahisa Sasho, Momoko Arai, Sota Kitahara, Yuichi Wada, Hideshige Moriya, and Kazuhisa Takahashi J Bone Joint Surg Am Volume 92(18):2909-2916 December 15, 2010 ©2010 by The Journal of Bone and Joint Surgery, Inc.

2 Histograms showing the concentrations of four basic growth factors (PDGF-AB, TGF-β1, VEGF, EGF) in platelet-poor clot releasates (PPCRs) and platelet-rich clot releasates (PRCRs) of four blood samples measured with enzyme-linked immunosorbent assay. Louay Fallouh et al. J Bone Joint Surg Am 2010;92:2909- 2916 ©2010 by The Journal of Bone and Joint Surgery, Inc.

3 Histogram showing anterior cruciate ligament cell viability (at twenty-four hours, forty-eight hours, and four days) as determined with WST-8 assay, which indirectly reflects the function of platelet-rich plasma treatment in promoting ACL cell viability. Louay Fallouh et al. J Bone Joint Surg Am 2010;92:2909- 2916 ©2010 by The Journal of Bone and Joint Surgery, Inc.

4 Histogram showing the total amount of collagen accumulated in the ACL cell layer during one week of monolayer culture with four different media: 5% fetal bovine serum (FBS), 5% platelet- poor clot releasate (PPCR), 5% platelet-rich clot releasate (PRCR), and... Louay Fallouh et al. J Bone Joint Surg Am 2010;92:2909- 2916 ©2010 by The Journal of Bone and Joint Surgery, Inc.

5 Histogram showing the gene expression levels of type-I collagen in ACL cells after two days of cultivation with 5% fetal bovine serum (FBS), 5% platelet-poor clot releasate (PPCR), and 5% platelet-rich clot releasate (PRCR). Louay Fallouh et al. J Bone Joint Surg Am 2010;92:2909- 2916 ©2010 by The Journal of Bone and Joint Surgery, Inc.

6 Histogram showing the gene expression levels of type-III collagen in ACL cells after two days of cultivation with 5% fetal bovine serum (FBS), 5% platelet-poor clot releasate (PPCR), and 5% platelet-rich clot releasate (PRCR). Louay Fallouh et al. J Bone Joint Surg Am 2010;92:2909- 2916 ©2010 by The Journal of Bone and Joint Surgery, Inc.


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