1. NCBI National Center for Biotechnology Information

2. Cytochrome c Oxidase subunits I and II Picture: http://www.ks.uiuc.edu/Research/bio_ener/cc o/ http://www.ks.uiuc.edu/Research/bio_ener/cc o/ Source: www.pdb.org/pdb/static.do?p= education_discussion/molecule_of_the_mont h/pdb5_1.htmln_discussion/molecule_of_the_mont h/pd Controls the last step in the oxidation of food. This enzyme add electrons and hydrogen to oxygen to form water.

3. http://www.ncbi.nlm.nih.govhttp://www.ncbi.nlm.nih.gov/ http://www.ncbi.nlm.nih.gov

5. Cytochrome c Oxidase subunit 1 mt

11. Clustalw2

12. Go back to your download. Copy and paste into this box.

14. Scroll down to Submit. Click, then wait.

20. Phylogram is a branching diagram (tree) assumed to be an estimate of a phylogeny, branch lengths are proportional to the amount of inferred evolutionary change. A Cladogram is a branching diagram (tree) assumed to be an estimate of a phylogeny where the branches are of equal length, thus cladograms show common ancestry, but do not indicate the amount of evolutionary "time" separating taxa. FROM EMBL-EBI

21. Go back to the download and annotate with the scientific name. Research and add the common name. Does this tree agree with what you assumed about these organisms?

22. Pan troglodyte- Chimpanzee Canis lupus familiaris- Dog Bos taurus-Cattle Mus musculus-Mouse Rattus norvegicus-Brown Rat Gallus gallus- Chicken Danio rerio-Zebrafish Drosophila melanogaster-Fruit Fly Anopheles gambiae-Mosquito Caenorhabditis elegans-nematode Schizosaccharomyces pombeFission Yeast Saccharomyces cerevisiae-budding Budding yeast Kluyveromyces lactis Lactose converting yeast Ashbya gossypii Filamentous fungus Arabidopsis thaliana Mouse ear cress Oryza sativa Indica Group-Rice Plasmodium falciparum-malaria protozoans

23. Choose your own proteins and organisms MVNFTAEEKTLINGLWSKVNVEEVGGEALGRLLVVYPWTHRFFDSFGNLSSASAIMGNPRVKAHGKKVLT AFGESIKNLDNLKSALAKLSELHCDKLHVDPENFKLLGNVLVIVLASHFGNEFTAEMQAAWQKLVAGVAT ALSHKYH >gi|57114340|ref|NP_001008890.1| hemoglobin, epsilon 1 [Rattus norvegicus] MVNFTAEEKSLINGLWSKVNVEEVGGEALGRLLVVYPWTQRFFDSFGNLSSASAIMGNPRVKAHGKKVLT AFGETIKNLDNLKSALAKLSELHCDKLHVDPENFKLLGNVLVIVLASHFGNEFTAEVQAAWQKLVAGVAT ALSHKYH >gi|52138683|ref|NP_001004390.1| hemoglobin subunit rho [Gallus gallus] MVHWSAEEKQLITSVWSKVNVEECGAEALARLLIVYPWTQRFFDNFGNLSSPTAIIGNPKVRAHGKKVLS SFGEAVKNLDNIKNTYAKLSELHCEKLHVDPENFRLLGNILIIVLAAHFTKDFTPTCQAVWQKLVSVVAH ALAYKYH >gi|71895591|ref|NP_001026660.1| epsilon globin [Gallus gallus] MVHWTAEEKQLITGLWGKVNVAECGAEALARLLIVYPWTQRFFASFGNLSSATAIIGNPMVRAHGKKVLS SFGEAVKNLDNIKKSFAQLSKLHCDKLHVDPENFRLLGDILIIVLASHFSKDFTPASQAAWQKMVRVVAH ALAHEYH >gi|49169791|ref|NP_990820.1| hemoglobin subunit beta [Gallus gallus] MVHWTAEEKQLITGLWGKVNVAECGAEALARLLIVYPWTQRFFASFGNLSSPTAILGNPMVRAHGKKVLT SFGDAVKNLDNIKNTFSQLSELHCDKLHVDPENFRLLGDILIIVLAAHFSKDFTPECQAAWQKLVRVVAH ALARKYH >gi|118085321|ref|XP_425673.2| PREDICTED: similar to Hemoglobin subunit epsilon (Hemoglobin epsilon chain) (Epsilon-globin) [Gallus gallus] MVHWSAEEKQLITSIWSKVNVEECGAEALARLLIVYPWTQRFFASFGNLSSPTAIMGNPRVRAHGKKVLS SFGEAVKNLDNIKNTYAKLSELHCDKLHVDPENFRLLGDILIIVLASHFARDFTPACQFAWQKLVNVVAH ALARKYH >gi|47086345|ref|NP_998011.1| hemoglobin beta embryonic-2 [Danio rerio] MVAWTAEERAFIQDIFSKLNYEEAGPKALQRALIVYPWTQRYFGSFGNLYNAEAIINNPKVAAHGTVVLH GLDRAMKNMDDIKNTYAELSVLHSEKLHVDPDNFRLLADCLTIVIASTMGAAFTADMQAAWQKFLAVVVS ALQRQYH >gi|61556716|ref|NP_001013045.1| ba1 globin, like [Danio rerio] MVEWTDAERTAILGLWGKLNIDEIGPQALSRCLIVYPWTQRYFATFGNLSSPAAIMGNPKVAAHGRTVMG GLERAIKNMDNVKNTYAALSVMHSEKLHVDPDNFRLLADCITVCAAMKFGQAGFNADVQEAWQKFLAVVV SALCRQYH >gi|18858329|ref|NP_571095.1| hemoglobin subunit beta-1 [Danio rerio] MVEWTDAERTAILGLWGKLNIDEIGPQALSRCLIVYPWTQRYFATFGNLSSPAAIMGNPKVAAHGRTVMG GLERAIKNMDNVKNTYAALSVMHSEKLHVDPDNFRLLADCITVCAAMKFGQAGFNADVQEAWQKFLAVVV SALCRQYH >gi|121583924|ref|NP_001073562.1| hemoglobin beta embryonic-like [Danio rerio] MVVWTDFEKATIQDIFAKADYDVIGPQALARCLIVYPWTQRYFAKFGNLYNAAAILGNPMVAAHGKTVLK GLELAVKNMDNIKATYADLSVLHSEKLHVDPDNFRLLADCLTIVVAAQMGAGFTPEVQAAFQKFIAVAVS ALGRQYH >gi|148236095|ref|NP_001091054.1| embryonic 1 beta-globin family member [Danio rerio] MVVWTDFEKATIQDIFAKADYDVIGPQALARCLIVYPWTQRYFAKFGNLYNAAAILGNPMVAAHGKTVLK GLELAVKNMDNIKATYADLSVLHSEKLHVDPDNFRVS >gi|62632719|ref|NP_001015058.1| hemoglobin beta embryonic-3 [Danio rerio] MVVWTAEERAAIQNIFAKLDFESVGLETLTRCLVVYPWTQRYFGGFGNLYNTEAIMANPKVKAHGVVVLK GLEKALNNMDNIKSTYASLSELHSEKLQVDPGNFRLLADCLTVVIATRMRSEFTPDIQAAWQKFLSVVVS ALRRQYL >gi|189517523|ref|XP_001918449.1| PREDICTED: hypothetical protein [Danio rerio] MVQWSDSERKTIASVWSKINVDEIGPQTLARVLVVYPWTQRYFGAFGDLSCASAIMGNPKVSEHGKTVLK ALEKAVKNVDDIKTTYAKLSQLHCEKLNVDPDNFKLLADCLSIVIATNFGPAFNPSVQSTWQKLLSVVVA ALTSRYF

25. Insulin is a good search

26. Copy the FASTA code

27. Into notepad Start with three proteins. Add one or two at a time. Hypothesize the relationship.

28. Check in ClustalW2