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1 SURVEY OF BIOCHEMISTRY Protein Function
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2 PRS In a protein, the most conformationally restricted amino acid is_____ and the least conformationally restricted amino acid is ________. 1. Trp, Gly 2. Met, Cys 3. Pro, Gly 4. Ile, Ala
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3 PRS In a protein, the most conformationally restricted amino acid is_____ and the least conformationally restricted amino acid is ________. 1. Trp, Gly 2. Met, Cys 3. Pro, Gly 4. Ile, Ala
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4 PRS The arrangement of the regular structural elements and the positions of atoms in the protein are considered part of the ______. 1. Primary structure 2. Secondary structure 3. Tertiary structure 4. Quaternary structure
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5 PRS The arrangement of the regular structural elements and the positions of atoms in the protein are considered part of the ______. 1. Primary structure 2. Secondary structure 3. Tertiary structure 4. Quaternary structure
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6 PRS Lysine can form a salt bridge by associating with a nearby ____ residue. 1. Pro 2. Ser 3. Gln 4. Glu
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7 PRS Lysine can form a salt bridge by associating with a nearby ____ residue. 1. Pro 2. Ser 3. Gln 4. Glu
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8 PRS Noncovalent forces that stabilize protein structure include all of the following except _______. 1. The hydrophobic effect 2. Salt bridges 3. Disulfide bridges 4. Metal-ion coordination
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9 PRS Noncovalent forces that stabilize protein structure include all of the following except _______. 1. The hydrophobic effect 2. Salt bridges 3. Disulfide bridges 4. Metal-ion coordination
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10 PRS Which of the following DNA sequences is (are) palindromic? 1. AGCT 2. AAGNCTT 3. AGGA 4. #1 and #2
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11 PRS Which of the following DNA sequences is (are) palindromic? 1. AGCT 2. AAGNCTT 3. AGGA 4. #1 and #2 5’-AGCT-3’ 3’-TCGA-5’ 5’-AAGNCTT-3’ 3’-TTCNGAA-5’ 5’-AGGA-3’ 3’-TCCT-5’
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12 Protein Function: Overview Transport Contraction Protection Catalysis Regulation –Gene regulation –Hormonal regulation Structural Support Chapter 7 - with emphasis on Myoglobin and Hemoglobin
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13 Why focus on Mb and Hb? Biological Importance
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14 Why focus on Mb and Hb?
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15 Why focus on Mb and Hb? Biological Importance Role in Transport of O 2 –Myoglobin: O 2 transport to muscles –Hemoglobin: O 2 transport from lungs Ligand Binding –Simple binding –Cooperativity and Allosteric Interactions
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16 Ligand Binding: General Concepts How can ligand binding be: –Described? –Measured? –Regulated? Distinctions between binding categories –One Protein + One Ligand –One Protein + Multiple Ligands
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17 Heme is what O 2 binds Heme is a porphyrin prosthetic molecule
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18 Myoglobin and Hemoglobin MyoglobinHemoglobin 1 4 8 8 Mb - O 2 Hb - O 2 # subunits # heme groups # Alpha Helices 18% identical residues
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19 Structure Changes on Binding Fig 6-38 shows Mb “breathing” How is O 2 binding to Mb described? Show on board…
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20 O 2 Binding to Myoglobin Shape indicates simple binding of O 2 to Mb
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21 Hb has 2 conformations Without O 2 T State With O 2 R State
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22 O 2 Binding to Hemoglobin Arteries carry oxygenated blood Veins carry deoxygenated blood
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23 Upcoming… More on Hb and Mb on Friday General concepts –Muscle Contraction –Antibodies Exam #1 on Thursday –Boggs B6A 4-5 pm
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