1. Ch 18: Protists

2. Protists unicellular eukaryotic

3. Prokaryotes vs Eukaryotes

4. Classification 115,000 species Major debate regarding how they should be classified “animal-like” = zooplankton or protozoans “plant-like” = phytoplankton 

5. Endosymbiotic Theory It is believed that protists evolved from a symbiotic relationship between different types prokaryotic cells Chloroplasts resemble cyanobacteria Mitochondria resemble aerobic bacteria endosym.theory tutorial animation

6. Photosynthetic prokaryote (Some cells) Chloroplast Mitochondrion Photosynthetic eukaryotic cell Aerobic heterotrophic prokaryote endosymbiosis generated mitochondria and chloroplasts Anaerobic-lacking oxygen; referring to an organism, environment or cellular process that lacks oxygen and may be poisoned by it Aerobic cells use oxygen to release energy from organic molecules by cellular respiration  -Proteobacteria Cyanobacteria

9. endomembrane system evolved from inward folds of the plasma membrane of a prokaryotic cell DNA Plasma membrane Cytoplasm Ancestral prokaryote Endoplasmic reticulum Nuclear envelope Nucleus Cell with nucleus and endomembrane system

10. Animal-like Protists 4 phyla: Ciliophora (ciliates) - Paramecium Zoomastigina (zooflagellates) Sporozoa – Parasitic - Plasmodium Sarcodina - ameoba

11. Phyla #1. Ciliophora – Ciliates Covered in cilia used for locomotion and for directing food into the oral cavity Most are free-living (not parasites)

12. Ex: Paramecium

13. Structure 1. Pellicle – cell membrane and underlay (looks quilt-like) controls entry and exit from the cell Embedded in the pellicle are the: 2. Trichocysts – spiny projectiles used for protection

14. 3. Macronucleus – controls cell’s activities 4. Micronucleus – used in conjugation to mix the genetic information

15. 5. Gullet (mouth). Cilia move food particles to the end of the gullet where they enter the cell by endocytosis. This traps the food particles into FOOD VACUOLES. The food vacuoles will fuse with LYSOSOMES (sacks of digestive enzymes) and be digested.

16. ENDOCYTOSIS

17. 6. Anal pore: Waste particles leave the cell by exocytosis at the ANAL PORE

18. Exocytosis

19. 7. Contractile Vacuoles Paramecium live in FRESH water Osmosis causes water to move into the paramecium The contractile vacuole collects the extra water so that the paramecium doesn’t lyse

20. Osmosis Review

21. Reproduction Like bacteria, paramecium reproduce mainly by BINARY FISSION

22. Paramecium Sex Again, like bacteria, paramecium will occasionally engage in conjugation – usually when they are stressed.

23. Conjugation is not actually “reproduction” – it is a trading and mixing of genetic material but no new paramecium are formed. However, new combinations of genes are created in both partners

24. Phyla #2: Zoomastigina Called the zooflagellates since they all move around using FLAGELLA Many are parasitic  Trichomonas causes intestinal and veneral diseases in humans

25. Giardia – causes “beaver fever”

26. Phyla #3: Sporozoa All produce spores All are parasites All non-motile Ex: Plasmodium (causes malaria) 

27. Sporozoa

28. Plasmodium Life Cycle 1. Spores transferred to humans through mosquito saliva 2. Spores grow in liver and blood cells, causing them to lyse, releasing toxins (creates fever, chills)

29. 3. Mosquitoes pick up Plasmodium in the blood when it bites the human 4. Mosquitoes become infected with Plasmodium – eventually, spores form in the salivary glands and the parasite is passed on to a new human

31. How to Avoid Malaria 1. Don’t go where there are malaria outbreaks 2. In areas where there is malaria, don’t get bit by mosquitoes 3. Chloroquinine will destroy some Plasmodium spores 4. Most often, countries with malaria try to control the mosquito host rather than the Plasmodium (insecticides and breeding ground removal)

32. Phyla #4: Sarcodina Use PSEUDOPODS to feed and move around

33. Ameoba