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Streptococcaceae II Jeanne Filbey MT(ASCP)

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Presentation on theme: "Streptococcaceae II Jeanne Filbey MT(ASCP)"— Presentation transcript:

1 Streptococcaceae II Jeanne Filbey MT(ASCP)
Division of Medical Technology Student Laboratory

2 Streptococcus viridans group
Alpha-hemolytic streptococci Streptococcus viridans group Colony morphology Tiny, gray, domed, alpha hemolysis Optochin/Taxo P resistant Bile solubility negative Bile esculin negative No growth in 6.5% NaCl

3 Streptococcus viridans group
Alpha-hemolytic streptococci Streptococcus viridans group Clinical significance Normal flora Generally non-pathogenic Subacute bacterial endocarditis (#1 cause) Liver abscesses, bacteremia Streptococcus anginosus/milleri group Deep wound infections

4 Abiotrophia species Nutritionally variant streptococci
Alpha-hemolytic streptococci Abiotrophia species Nutritionally variant streptococci Requires Vitamin B6 / pyridoxal to grow Clinical significance Normal flora Endocarditis

5 How can you differentiate Streptococcus pneumoniae from Streptococcus viridans group?
Susceptibility to Optochin and bile solubility. Streptococcus pneumoniae is optochin sensitive and bile solubility positive. Streptococcus viridans group is optochin resistant and bile solubility negative.

6 Streptococcus viridans group are clinically significant for what disease?
Subacute bacterial endocarditis (#1 cause)

7 Abiotrophia species are clinically significant for what disease?
Subacute bacterial endocarditis

8 Gemella species Colony morphology Gram stain: GPC in pairs and chains
Streptococcus-like bacteria Gemella species Colony morphology Resemble Streptococcus viridans group Can be alpha or gamma hemolytic Gram stain: GPC in pairs and chains Easily decolorized Pairs may have adjacent sides flattened

9 Gemella species Identification Streptococcus-like bacteria
PYR variable Vancomycin sensitive Glucose non “F” Bile esculin negative No growth in 6.5% NaCl

10 Gemella species Clinical significance Streptococcus-like bacteria
Normal flora of respiratory and GI tracts Rarely isolated from humans Opportunistic pathogen Septicemia Wounds, abscesses Respiratory UTI

11 Leuconostoc species Colony morphology
Streptococcus-like bacteria Leuconostoc species Colony morphology Resemble Streptococcus viridans group Can be alpha or gamma hemolytic Gram stain: GPC in pairs and chains Can be coccobacilli in appearance

12 Leuconostoc species Identification Streptococcus-like bacteria
PYR negative Vancomycin resistant Glucose “F” Bile esculin positive Variable growth / no growth in 6.5% NaCl

13 Leuconostoc species Clinical significance Streptococcus-like bacteria
Normally found in environment Opportunistic pathogen in immunosuppressed patients Septicemia Wounds CSF

14 Pediococcus species Colony morphology
Streptococcus-like bacteria Pediococcus species Colony morphology Resemble Streptococcus viridans group Can be alpha or gamma hemolytic Gram stain: GPC in pairs, tetrads and clusters

15 Pediococcus species Identification Streptococcus-like bacteria
PYR negative Vancomycin resistant Glucose “F” Bile esculin positive Variable growth / no growth in 6.5% NaCl Group D antigen positive

16 Pediococcus species Clinical significance Streptococcus-like bacteria
Normally found in environment Opportunistic pathogen in immunosuppressed patients Blood Saliva Stool Urine Wounds

17 How can you differentiate Gemella, Leuconostoc and Pediococcus from each other?
Biochem: G.S. PYR Van Gluc BE 6.5NaCl Gemella Pair, chain Variable Sensitive Non “F” Negative No growth Leuconostoc Resistant “F” Positive Pediococcus Tetrad, cluster Type Grp D

18 Summary… Classification – hemolysis, C-substrate
Gram stain – GPC in pairs & chains Identification Beta Strep (PYR, Taxo A, SXT, Na hipp) Alpha Strep (Optochin, bile solubility) Gamma Strep (PYR, BE, 6.5% NaCl) Strep-like organisms Clinical significance of each organism

19 Who am I? Gram stain BAP PYR Enterococcus species

20 Streptococcus pyogenes
Who am I? Gram stain BAP PYR Streptococcus pyogenes

21 Streptococcus pneumoniae
Who am I? Gram stain BAP with optochin Streptococcus pneumoniae

22 Streptococcus agalactiae
Who am I? PYR Na hippurate Gram stain BAP Streptococcus agalactiae

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