1. Bio-Rad Science Ambassador Program Your profession is your passion. Pass it on. bio-rad.com/scienceambassadors

2. Bio-Rad Science Ambassador Program 2  Focuses on isolating your own DNA from cheek cells  The DNA will be transferred into an amulet  The amulet will be a permanent keepsake of your genetic material — your personal essence! Today’s Activity Genes in a Bottle Activity

3. Bio-Rad Science Ambassador Program 3 Timeline Genes in a Bottle Activity Introduction Background on DNA Extraction Extract Genomic DNA from Cheek Cells Prepare DNA Necklaces

4. Bio-Rad Science Ambassador Program 4 What is DNA?

5. Bio-Rad Science Ambassador Program 5 what can you see? If you pull out one of your hairs,

6. Bio-Rad Science Ambassador Program 6 Hair cells are found in the root of the hair

7. Bio-Rad Science Ambassador Program 7 Hair cells have information about your hair

8. Bio-Rad Science Ambassador Program 8 What are the parts of an animal cell? A Cell

9. Bio-Rad Science Ambassador Program 9 Cells come in all shapes and sizes All have DNA!

10. Bio-Rad Science Ambassador Program 10 DNA is inside cells Cells are liken bags that hold DNA Break open the bags and out comes the DNA!

11. Bio-Rad Science Ambassador Program 11 Protocol Highlights Step 1: Use a simple water mouth wash to collect cheek cells Step 2: Add lysis buffer to cells to break open cell and nuclear membranes and release nuclear contents Step 3: Precipitate DNA with cold alcohol in high salt Step 4: Transfer your DNA into the amulet

12. Bio-Rad Science Ambassador Program 12 Illustrated Guide of Kit Components Plastic transfer pipet Micro test tube 15ml conical tube Amulet

13. Bio-Rad Science Ambassador Program 13 Zip-Top Bag Components Workstation Inventory 15 ml conical tube 1 Colored micro test tube with protease/salt/lysis buffer1 Disposable plastic transfer pipet1 Necklace components1 Tattoo1 Small paper cup1

14. Bio-Rad Science Ambassador Program 14 Step 1: Cheek Cell Collection In this step, you will collect your cheek cells in a 15 ml conical tube using a water mouth wash. 1.Take out the 15 ml conical tube which contains 3 ml of water and label with your initials. 2.Practice using the transfer pipet by transferring 1 ml of water from a large cup to your small cup. Repeat 3 times for a total of 3 ml of water. 3.Take the water from the small cup into your mouth — don’t swallow it! Swish the water around like mouthwash and gently chew on the inside of your cheeks while you swish the water around for 30–60 seconds. 4.Carefully expel the water back into the small paper cup. Pour the water-cheek cell mix back into your 15 ml tube.

15. Bio-Rad Science Ambassador Program 15 Step 2: Cell Lysis and Proteolysis In this step, you will lyse your cheek cells and degrade the protein with a protease. 1.Open the colored micro test tube containing a protease/salt/lysis buffer mixture. Pour the entire contents into the 15 ml conical tube containing your cheek cells. 2.Place the cap on the tube and gently invert it 5 times — don’t shake it! Observe your tube — do you notice any changes? 3.Incubate for 5 minutes by warming the tube in your hand. This allows time for the protease to break down the proteins.

16. Bio-Rad Science Ambassador Program 16 Step 3: DNA Precipitation In this step, you will precipitate your DNA with the addition of alcohol. 1.Hold your tube at a 45°angle and slowly fill the tube with alcohol by gently pouring or pipetting alcohol down the inside wall of the tube. Fill the tube to the 14 ml mark. 2.Cap your tube and let stand undisturbed for 5 minutes at room temperature. What do you see? (You should begin to see bubbles and white strands appearing at the interface between the alcohol and water phases — this is your DNA!) 3.Very gently tilt the tube on its side and then turn it upright about 10 times until the water and alcohol phases are mixed. 4.Once mixed, the DNA should be fully visible as a “precipitate”.

17. Bio-Rad Science Ambassador Program 17 Step 4: Necklace Assembly In this step, you will assemble your DNA necklace. 1.Place the amulet on your desk. Using a plastic pipet, carefully transfer as much of your DNA precipitate and only as little alcohol as you can into the amulet. The amulet requires approximately 0.5 ml to fill. 2.Screw the cap onto the amulet. 3.Slip the waxed cord through the cap. Your DNA necklace is now complete!

18. Bio-Rad Science Ambassador Program 18 Congratulations! Genes in a Bottle ™ Kit You have just created your very own DNA necklace!

19. Bio-Rad Science Ambassador Program 19 How long does the DNA in the necklace last? The DNA in the amulet can last for years. Add more alcohol into the vial if some evaporation occurs. Bulletin 6378 Rev A 13-0498 0313