1. California’s Pierce’s Disease Control Program Bob Wynn Statewide Coordinator - November 2014 -

2. Background 1999: “Say goodbye to California wines.”

3. GWSS in California June 2002

4. Containment Nursery Bulk Citrus Bulk Grape Prevent GWSS from moving to new areasPrevent GWSS from moving to new areas

5. Containment Program Totals Nursery shipments818,200Nursery shipments818,200 Nursery rejections597Nursery rejections597 Citrus shipments732,525Citrus shipments732,525 Citrus rejections252Citrus rejections252 Nursery ATP Program 8 nurseries (28 yards)8 nurseries (28 yards) Approx. 20% of nursery shipments are from ATP nurseriesApprox. 20% of nursery shipments are from ATP nurseries

6. Statewide Survey Verify that at-risk areas are still uninfested Look for new/unknown infestations of GWSS Currently being conducted in 49 counties (6 generally infested, 36 uninfested, 7 partially infested) Approximately 38,000 traps deployed in nursery and urban/residential areas during peak season

7. Rapid Response Recent Highlights GWSS infestations eradicated in San Luis Obispo and Santa Clara County GWSS trapped in Alameda, Fresno, Imperial, Santa Clara, and Tulare County

8. Biological Control Program Totals 7 species released 15 counties covered 172 release sites 2.48 million agents released Recent Activity Arvin production facility (2 greenhouses, 5,000 sq ft) 3 species being released Releases in Fresno, Kern, Madera, Santa Barbara, Tulare, and Ventura County

9. Area-wide Projects Coordinated vector control over large agricultural areas

10. Area-wide Projects

11. - Funds requested for FY 2015 - Kern County$1,000,000 Tulare County$580,000 Fresno County$323,971 Madera County$143,736 $2,047,707

12. Past & Present Then Now

13. Research Over 200 projects conducted since 1999 Symposium held 12 years; 915 reports in 13 years of Proceedings Next Symposium will be held Dec. 15-17, 2014 in Sacramento Field trials in progress (conventionally-bred & transgenic methods)

14. Commercializing Promising Technologies Objectives PD protection to growers as soon as possiblePD protection to growers as soon as possible Insurance against future, devastating outbreaksInsurance against future, devastating outbreaks Protect California grape industryProtect California grape industry Protect National grape industryProtect National grape industry

15. Technology Facilitator Began in July 2013 Providing advice and guidance on the development and commercialization of promising discoveries and technologies Serves on research-related committees

16. 14 Years  Technology & Product Pipeline Conventional Breeding Ongoing Breeding Program Protection from Transgenic Strategies Ongoing Science

17. Technology Inventory Walker: Breeding Back-cross Marker assisted breeding Cultivars and Rootstocks Walker: Breeding Back-cross Marker assisted breeding Cultivars and Rootstocks Lindow: DSF spray, competitive endophytes Rolshausen: fungal & bacterial endophytes, natural products Lindow: DSF spray, competitive endophytes Rolshausen: fungal & bacterial endophytes, natural products Kirkpatrick: Hemagglutinin Powell, Labavitch, Dandekar: pPGIP Gilchrist: PR1 and ut456 Lindow: DSF Dandekar: CAP Powell, Labavitch, Dandekar: pPGIP Gilchrist: PR1 and ut456 Lindow: DSF Dandekar: CAP Conventional Transgenic Early Field Trials Lab Research Greenhouse Advanced Field Trials, Multi-State UCD Foundation Plant Services Walker: 5 PdR sequences Dandekar: grape binding & lytic domains Falk: siRNA Hammock: juvenile hormone Burr : Xf Δ1311 biocontrol Lin: gcvR gene (biofilm) Walker: 5 PdR sequences Dandekar: grape binding & lytic domains Falk: siRNA Hammock: juvenile hormone Burr : Xf Δ1311 biocontrol Lin: gcvR gene (biofilm)

18. Walker Breeding Program: Current Cultivars, Ongoing Pipeline Cultivars advanced to FPS March, 2013: 15 cultivars: seven 94%, eight 97% vinifera Three rootstocks All PdR1: from b43-17 cross with V. arizonica Last vinifera parent: Cabernet, Zin, Chardonnay, Petite Sirah, Lacryma Christi, Cab x Carignane Earliest release to nurseries: 2015; additonal 3+ years to growers Cultivars advanced to FPS March, 2013: 15 cultivars: seven 94%, eight 97% vinifera Three rootstocks All PdR1: from b43-17 cross with V. arizonica Last vinifera parent: Cabernet, Zin, Chardonnay, Petite Sirah, Lacryma Christi, Cab x Carignane Earliest release to nurseries: 2015; additonal 3+ years to growers Current field trials: CA: Davis, Napa: mostly 88%, 94%, some 97% TX: 3 sites; 88%, 94% vinifera AL: 1 site FL: potential 1 site Current field trials: CA: Davis, Napa: mostly 88%, 94%, some 97% TX: 3 sites; 88%, 94% vinifera AL: 1 site FL: potential 1 site Ongoing pipeline: Additional resistance genes: Southeast US, Mexico: V. shuttlworthii, BD5-117 Multi-gene resistance, complex crosses Advanced materials for continued breeding Adding powdery mildew resistance, salt/drought resistance Ongoing pipeline: Additional resistance genes: Southeast US, Mexico: V. shuttlworthii, BD5-117 Multi-gene resistance, complex crosses Advanced materials for continued breeding Adding powdery mildew resistance, salt/drought resistance Cultivars expected to be advanced to FPS May, 2014: ~10 cultivars: 97% vinifera 97%: Cabernet Sauvignon, Zin, Chardonnay, Sylvaner: early, mid-early, mid, late Earliest release to nurseries: 2016; additional 3+ years to growers

19. Transgenic Strategies Gum ‘em up: Kirkpatrick: Hemagglutinin Lindow: DSF Gum ‘em up: Kirkpatrick: Hemagglutinin Lindow: DSF Search and destroy: Dandekar: chimeric bind & lyse Search and destroy: Dandekar: chimeric bind & lyse Grape life support: Gilchrist: PR1 and UT456 Grape life support: Gilchrist: PR1 and UT456 Passport denied: Powell, et. al.: PGIP Passport denied: Powell, et. al.: PGIP

20. Viticulture Consultant Began in February 2014 Providing advice and guidance on the viticultural practices followed in the field trials Advises on other research-related activities Serves on research-related committees

21. Field Trials

22. Untransformed Thompson Seedless Transformed Thompson Seedless Field Trials

23. Other Designated Pests & Diseases Beginning in 2010, assessment funds may be spent for research and outreach on other pests and diseases of winegrapes Pest or disease must be formally designated To formally designate, specified findings must be made Currently designated: European grapevine moth Brown marmorated stink bug Red blotch Vine mealybug

24. AB 1642 Introduced February 2014 by Assembly member Chesbro et al.Introduced February 2014 by Assembly member Chesbro et al. Signed by Governor in August 2014Signed by Governor in August 2014 Continues the Pierce’s Disease Control Program, PD/GWSS Board, winegrape assessment, and PD Advisory Task Force for another five yearsContinues the Pierce’s Disease Control Program, PD/GWSS Board, winegrape assessment, and PD Advisory Task Force for another five years Continuation of Board and assessment is contingent upon favorable grower vote, to be held this SpringContinuation of Board and assessment is contingent upon favorable grower vote, to be held this Spring

25. Thank you! ---Questions?