1. Lab 11& 12 Biochemical Characterization Practice questions Designed by Pyeongsug Kim ©2010 webmaster@science-i.comwebmaster@science-i.com Fall 2011 Chattahoochee Technical College Picture from http://www.midlandstech.edu/science/kelleherk/225/labmaterials/sel_diff_media.htmlhttp://www.midlandstech.edu/science/kelleherk/225/labmaterials/sel_diff_media.html

2. Designed by Pyeongsug Kim, ©2010 www.science-i.comwww.science-i.com What is the name of each medium? Identify whether each agar is selective or differential. Picture from http://www.cfkeep.org/html/stitch.php?s=72880656991974&id=24286258057026http://www.cfkeep.org/html/stitch.php?s=72880656991974&id=24286258057026 D MSA agar – both selective and differential TSA agar – Neither Blood agar – Differential EMB agar – both selective and differential A: B: C: D:

3. Designed by Pyeongsug Kim, ©2010 www.science-i.comwww.science-i.com What is the name of the media above? Is selective or differential media? Explain your answer. Is it shown positive result of negative result in each medium? A: B: C: Picture from http://archive.microbelibrary.org/ASMOnly/details.asp?id=2553&Langhttp://archive.microbelibrary.org/ASMOnly/details.asp?id=2553&Lang A B C EMB agar Both selective and differential Selective ~inhibit the growth of Gram positive bacteria (selective for Gram negative) Differential ~ differentiates coliforms (Lactose fermenters) Positive (lactose fermentation, coliforms) Positive (Lactose fermentation, coliforms) Negative What is the name of the media above? Is selective or differential media? Explain your answer.

4. Designed by Pyeongsug Kim, ©2010 www.science-i.comwww.science-i.com AA Is it shown positive result of negative result in each medium? A: B: What is the name of the media above? Is selective or differential media? Explain your answer. MSA plate Both selective and differential Selective ~Selective for Gram positive and Salt tolerant Differential ~ differentiates for mannitol fermentation Positive (manitol fermentation) Negative Picture from http://lifesci.rutgers.edu/skelly/images/Msa%20Plates.htmhttp://lifesci.rutgers.edu/skelly/images/Msa%20Plates.htm

5. What results are shown? A: B: C: Picture from http://iws2.collin.edu/dcain/CCCCD%20Micro/hemolysis.htmhttp://iws2.collin.edu/dcain/CCCCD%20Micro/hemolysis.htm Designed by Pyeongsug Kim, ©2010 www.science-i.comwww.science-i.com What is the name of the test above? What is the name of media above? Is selective or differential media? Explain your answer. Hemolysys test Differential ~ differentiates for hemolysis Alpha hemolysis Gamma hemolysis Beta hemolysis Blood agar AB C B

6. Designed by Pyeongsug Kim, ©2010 www.science-i.comwww.science-i.com What properties of bacterial metabolism are demonstrated above? A: B: C: Is selective or differential media? Explain your answer. Sugar fermentation Differential ~ differentiates for sugar fermentation Positive ~ Lactose fermentation (acid/ No gas) Positive ~ Dextrose fermentation (acid/gas forming) Negative for maltose fermentation (No acid/ No gas) Phenol red broth with sugar Each tube contains …. A: Lactose B: Dextrose C: Maltose. A B C Picture from http://a-s.clayton.edu/furlong/BIOL3250/lab/Review/page/biochemicaltestanswer.htmhttp://a-s.clayton.edu/furlong/BIOL3250/lab/Review/page/biochemicaltestanswer.htm What is the name of media?

7. Semisolid media What properties of bacterial metabolism are demonstrated above? A: B: A B S Positive for Hydrogen Sulfide production S Negative for Hydrogen Sulfide production Designed by Pyeongsug Kim, ©2010 www.science-i.comwww.science-i.com What is the name of media? Is it selective or differential? SIM -Differential A B What name of the media is shown? Is it selective or differential? SIM; Differential What properties of bacteria is shown? A: B: Motility Nonmotile Motile Picture from http://inst.bact.wisc.edu/inst/index.php?module=book&func=displayarticle&art_id=118http://inst.bact.wisc.edu/inst/index.php?module=book&func=displayarticle&art_id=118

8. Designed by Pyeongsug Kim, ©2010 www.science-i.comwww.science-i.com Kovac’s reagent What is the name of media? Is it selective or differential? SIM; Differential What name of the test is? Indole test AB Control What biological prosperity do this bacteria have? A: B: Negative for indole production Positive for indole production Picture from http://subota.svetlostistine.org/negative-indole-test&page=6http://subota.svetlostistine.org/negative-indole-test&page=6

9. What is the name of media? Is it selective or differential? Designed by Pyeongsug Kim, ©2010 www.science-i.comwww.science-i.com Methyl red Picture from http://www.mesacc.edu/~johnson/labtools/Dbiochem/imvic.htmlhttp://www.mesacc.edu/~johnson/labtools/Dbiochem/imvic.html What is the name of the test(left)? MR-VP; Differential What biological prosperity do this bacteria have? A: B: A B Negative for Mixed acid production Positive for Mixed acid production VP test What is the name of the test(left)? What biological prosperity do this bacteria have? A: B: MR test Negative for acetoin production Positive for acetoin production

10. Designed by Pyeongsug Kim, ©2010 www.science-i.comwww.science-i.com Picture from: http://web.fccj.org/~lnorman/unknowns.htm?index=2http://web.fccj.org/~lnorman/unknowns.htm?index=2 A B Before putting the reagent. After putting the reagent. What is the name of the test(left) using a starch agar? What biological prosperity do this bacteria have? A: B: Extracellular amylase test Negative-No amylase presence Positive - Amylase presence What is the name of the reagent that used in this test? Gram-Iodine Which one, A or B, is shown starch presence? A

11. Designed by Pyeongsug Kim, ©2010 www.science-i.comwww.science-i.com What is the name of the test(left)? Hydrogen peroxide Picture from http://iws2.collin.edu/dcain/CCCCD%20Micro/catalase_test.htmhttp://iws2.collin.edu/dcain/CCCCD%20Micro/catalase_test.htm Catalase test What type of reagent is using for this test? What biological prosperity do this bacteria have? A: B: Negative – Catalase presence. Positive – Catalase presence