1. Artemia culture in Southeast Asia: Special emphasis to Vietnam By: Nguyen Thi Hong Van and Nguyen Van Hoa Aquaculture and Fisheries Sciences Institute Cantho University

2. - In most of the countries that belong to Southeast Asia like Philippine, Malaysia, Indonesia…ect Artemia culture in the salt-pan presently are not yet done except indoor culture in the hatcheries - In Thailand, there are a few places that culturing Artemia biomass for ornamental fish industry and exporting to the Singapore. - Artemia culture indoor in most of the hatcheries in Southeast Asia not only used as live food for shrimp and fishes but also used as a bio-filter to clean the seawater

3. In Viet Nam, on the year 2001, the Artemia cultured area declined more than 50% compared to last year since the cyst price loosed about ¼ and most of farmers switched into salt production

4. Large Production This activity was done mainly under the aid of AFSI extension team (est. 60ha). Most of remaining cultured area were self-management by experienced farmers (est. 360 to 370ha). - The yields varied from 20kg to more than 100kg/ha/crop - Average yields: 45kg/ha/crop

5. Extension activities of AFSI Training at AFSI Transporting nauplii to farmers

6. Advantages: Culture technique were gradually transferred to the farmers and the culture area are expanding by time. To decrease the working pressure on extension team when the culture area are expanded. Disadvantages: Less experienced or new farmers normally have poor production. Farmers usually manage the production by their feeling and experiences rather than their understanding on biological processes that appear in the ponds.

7. Some characteristics on management of large production: - Salinity: 70 ppt upwards - Water level: normally shallow (2-3cm at the beginning and 5-10 at the end of the culture) - Stocking densities: high: 200-300 nauliar/litter. Farmers prefer to stock with very high densities and after few days the densities were lowered by transferring Artemia to another pond. * Problem: - Water levels are very shallow, shortage of food - Salinity are slowdown dramatically - Copepode population grow quickly - Macro-algae and Lab-lab development Survival is very low

8. - Fertilized pond are not well management - Heavily rely on supplement food: Rice bran, chicken manures Lab-lab Seaweed Chicken manures

9. Research at AFSI Sampling Observation Collected samples Alga culture Turbid measure Prepare for sampling

10. Research 1.Wild algae as food for Artemia. 3 algae species are used to test on growth and survival of Artemia: + Chaetoceros  Best growth and survival + Nizchia  can survive but slow growth + Oscillatoria  no growth, died after few days

11. 2. Control problem of ‘water bloom’ in Artemia pond. Water bloom (algae bloom) appear Pond with poor organic matters Ponds with rich organic matter Less Always Stocking densities High yields 60-70ind/l 120-150ind/l

12. 3. Comparison life characteristics of Artemia between field and lab conditions Water flow A: bottle coverred with net at 3 sides B: rack for individual culture in pond C: Rack was hung in the water column of the culture ponds Figure 2: individual culture system in pond

13. + Pair culture in the laboratory: The animals were fed with water from the culture pond, the culture medium was maintained similar as in the pond Results : The reproductive characteristics (reproductive mode, fecundity) - Pond culture (sampling) - Pair culture in pond - Pair culture in the laboratory No sinificant differences

14. 4. Effect of using Artemia nauplii of successive generations on cyst production - Objective: Investigating the relationship of cyst yields and successive generations (P, F1, F2, F3) - Principle: Inoculum (P=cysts) 14 days Naupliar transfer F1 F2 F3 14 days Naupliar transfer

15. Result: - Survivals: After 7days of the inoculation: 50-60% After 9-10 days (reach adult sizes): 25-40% The major reason for this decline could be the food availability in pond and environmental stress (especially is high temperature). - Reproductive characteristics: + Fecundity: varies from 30 to more than 100 offspring and not much different between generations + Number of female - bearing nauplii do not follow any rule but number of female-bearing cysts are follow the same rule for each generation.

19. The cyst yields: (kg/ha/5week) 1999-2000 2000-2001 2001-2002 Parent 60.0653.01 24.58 F168.8446.2 5.43 F281.0720.98 28.75 F3 12.47 The cyst yields were largely changed over years, beside the unknown factors that need to be investigated (environment stress, genetics…). Other factors also might influence to cyst production included:

20. + Seasonal reproduction of Artemia + Food availability + Temperature Most importance Future research Suitable pond management fit to different kind of pond bottom soil, especially emphasizing to the proper stocking densities and fertilization. Effects of biotic factor (food availability), abiotic factor (temperature) to reproductive characteristics and cyst production of successive generations Effect of quality and quantity of food (algae) on Artemia cyst production of successive generations. Genetic variation and heritable traits of Artemia between generations (P, F1, F2, F3).

21. Thanks for your attention