1. Faculty of Allied Medical Science
Blood Banking (MLBB 201)

2. HAEMOSTATIC COMPONENTS
Prof.Dr. Nadia Aly Sadek
Director of Blood Bank Centre – MRI
University of Alexandria

3. ILOs
By the end of this lecture, the students will be able to recognize;
What is Fresh frozen plasma and cryoprecipitate.

4. Content

5. Fresh Frozen Plasma (FFP)1
Fresh Frozen Plasma (FFP)
Prepared from whole blood donation and frozen within 6 – 8 hours .
It is stored at – 18o c or colder for up to 12 months.
It contains: 91% water – 7% protein – 2% carbohydrate.

6. Fresh Frozen Plasma (FFP)1
Fresh Frozen Plasma (FFP)
Indications
To treat prolonged PT or PTT
Vitamin K deficiency and liver disease
Dilutional coagulopathy
Protein S, Protein C or Antithronbin deficiency.

7. Fresh Frozen Plasma (FFP)1
Fresh Frozen Plasma (FFP)
Thawing
It should be thawed at temperature 30-37oc in a water bath.
The unit should be wrapped in a plastic bag.
Thawed FFP should be transfused immediately or within 12 hrs or stored between 1-6oc for no more than 12 hours.
It should not be refrozen.

8. Fresh Frozen Plasma (FFP)1
Fresh Frozen Plasma (FFP)
Administration
The same ABO as the protein.
Given via a filter over 1 – 1.5 hours.

9. CRYOPRECIPITATE 2 Obtained from FFP and concentrated to 10 - 20 ml.
It contains F VIII, (80 – 120 Units), v WF, 250 mg fibrinogen, 20 – 30% of factor XIII & fibronectin.

10. CRYOPRECIPITATE 2 Indications Factor VIII deficiency (Haemophilia A).
Type II VW disease.
Type I VW disease not responding to DDAVP.
Factor XIII deficiency.
Congenital fibrinogen abnormality.

11. CRYOPRECIPITATE 2 Indications When fibrinogen is ≤ 100 mg.
Control of bleeding in uremic or hepatic patients.
For preparation of fibrin glue: Topical use in orphopedic, ENT, dental, cardiac or neurological procedures.
Reversal of warfarin overdose.

12. CRYOPRECIPITATE 2 Thawing
It should be thawed at 37 0 c immediately before use and the pack should NOT be refrozen.
Fibrinogen dose: 8 – 10 bags supply 2 g fibrinogen.
Infusion
Filter at rate of 1 – 2 ml/min.

13. 2
CRYOPRECIPITATE
Cryoprecipirate represents the cryoglobulin fraction of the plasma.
It contains F VIII, VWF, fibrinogen, fibronectin and F XIII.
It is used primarily for the treatment of Haemophilia A patients.

14. Platelet Concentrates3
Platelet Concentrates
Volume: 50 – 70 ml/unit provide about platelets. Kept at 20 – 22 0 c in a shaker for a maximum of 5 days.
Should be infused within 4 hours, administered with filter

15. 4
Prothrombin Complex
This component contains prothrombin (F II), factor VII, F IX and factor X.
It is lyophylized and virus-inactivated to reduce the risk of transmitting infections.
Indications
Haemophilia B (F IX deficiency).
Serious coumarin overdosage together with Vitamin K1
Coagulation defects due to fulminant hepatitis or liver insufficiency.

16. 5
FEIBA AND AUTOPLEX
These are some activated prothrombin complex preparations used for treatment of Haemophilia A patients with inhibitors.
These should be given with caution, as overuse may cause thrombosis and DIC.
Currently, there are factors IX and F VII concentrates for TR of Haemophilia B.

17. 6
Fibrin Glue
It is formed by the reaction of cryo (fibrinogen) + thrombin as they are applied topically.
Advantage
Good hemostasis in different solid organ bleeding.
Prevents post-operative bleeding.
Efficient in the presence of coagulation defect and bacterial contamination.

18. Study Question
Mention the indication of cryopercipitate.

19. Assignments
Complication of blood transfusion
محمد مصطفى محمد

20. THANK YOU