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Published byNoreen Harris Modified over 9 years ago
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Use of OPU-IVP and SO at CRV breeding program in the genomic era
Erik Mullaart and Sybrand Merton
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Impact on breeding program
Holland Genetics Content Introduction Genomic Selection Impact on breeding program Selection procedures Delta Nucleus breeding program Application of OPU-IVP and SO Insire embryo program
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CRV Worldwide
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Genetic products of CRV
7 million units of dairy and beef semen CRV exports semen to over 50 countries Test program: 310 Holstein bulls 60 Red Holstein bulls 35 Fleckvieh bulls 35 Jersey bulls
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Genomic selection
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Which calf received the best genes from the parents?
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Marker selection In early days: selection on individual genes (direct or via markers) Now: selection based on many markers spread across the whole genome, i.e. selection on all genetic material =GENOMIC SELECTION (or Genomewide Selection)
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Genomic Selection, What does it bring us?
Reliable breeding values at young age higher success rate to get top bulls lower costs for test proofs (60,000 markers for ~ € 200) shorter generation-interval Toelichting geven
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Effects of Genomic Selection
Increased genetic improvement (30-40%) Higher genetic level of test sires and embryo’s Wider portfolio of commercial bulls & higher level More outcross; less inbreeding Availability of packs of elite young sires (InSire)
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Impact on breeding program; selection procedures
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Use of GS in selection procedures; method implemented by CRV
Step 1: Genotype proven bulls with reliable EBVs (reference sires) Find markers with an effect on EBVs Calculate marker effect for each trait Step 2: Genotype selection candidates Calculate GS Breeding Values (GBVs) with the marker effects Select the animals with the highest GBVs
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3 full sisters on Delta. Pedigree ( Oman x Jocko Besne)
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Impact on breeding program; Delta nucleus breeding program
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16 years of ‘Delta nucleus’
Test heifers 4381 Selected donors 561 Before Genomic Selection large number of test heifers per year emphasis on independent test With Genomic Selection. limited number of test heifers optimal use of reproductive possibilities
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After GS-selection 110 donor heifers
Delta nucleus Selection of 800 calves GS selection After GS-selection 110 donor heifers MOET en OPU embryos per heifer 100 selected heifers tested Selection of donor cows ET en OPU => 2500 embryos
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Application of OPU-IVP and SO
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Embryo production IVP sessions: 2000 / year SO: 300 / year
1.6 embryo/ session SO: 300 / year 4-5 embryos/ flush
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Production Logistics 3 x SO at 13-17 months of age: 12 embryos
Holland Genetics 3 x SO at months of age: 12 embryos Inseminate at 17 months 17 x OPU at months: 20 embryos Calving at 26 months 32 embryos
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Future Production Logistics
Holland Genetics 2 x SO at months of age: 8 embryos 10 x OPU at months: 12 embryos Inseminate at 18 months 17 x OPU at months: 20 embryos Calving at 27 months 40 embryos
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Working method: SO D -12 --- Start protocol FSH 1x D -11 --- FSH 2x
D FSH 2x + Prostaglandin with 5th FSH injection D FSH 2x + Prostaglandin with 6th FSH injection D FSH 1x D Heat + AI (2x 8-12 hr / hr) D Flush + Prostaglandin + Progesterone application D Remove PRID D Heat D D start SO again (D - 12)
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Working method: OPU-IVP
Day -1 Ovum Pick Up (OPU) In vitro maturation (IVM) Day 0 In vitro fertilization (IVF) Day 1 Start in vitro culture (IVC) Day 4 Cleavage evaluation Day 6 Prognoses embryo production Day 7 Embryo evaluation (fresh, frozen) Day 8 Final embryo evaluation (frozen)
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Working method: OPU OPU twice a week (Mo, Thu) No superstimulation
Holland Genetics Working method: OPU OPU twice a week (Mo, Thu) No superstimulation (para)veterinarian plus assistant Pregnant heifers and first parity donor cows
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Working method: IVM Maturation for 16 - 28 h, 38.5°C, 20% O2
Holland Genetics Working method: IVM Maturation for h, 38.5°C, 20% O2 Volume: transport and maturation in 0.5 ml in tubes
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Working method: IVF Semen: Frozen-thawed
Holland Genetics Working method: IVF Semen: Frozen-thawed Separation semen and extender by gradient centrifugation (90% / 45%) Volume: wells (0.5 ml), Incubation for 24 h, 38.5 °C, 20% O2
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Working method: IVC Medium: SOFaaBSA Volume: wells (0.5 ml)
Holland Genetics Working method: IVC Medium: SOFaaBSA Volume: wells (0.5 ml) Incubation for 6-8 days, 38.5°C, 5% O2
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Working method: IVC Embryos Day 7 - 8, fresh / frozen Stage 4 - 9
Holland Genetics Working method: IVC Embryos Day 7 - 8, fresh / frozen Stage 4 - 9 (Morula - Hatched Blastocyst) Grade 1 and 2 IETS
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Working method: Embryo transfer
Holland Genetics Recipients Healthy, fertile recipients Natural cycling Day 6, 7, 8 or 9 of estrus cycle <5% rejected recipients 150 contracted farms
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Working method: Logistics OPU / IVP
Holland Genetics Working method: Logistics OPU / IVP OPU and SO location Transport COC’s Central IVP lab Transport of fresh embryos Recipient herds Farmers
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Results: pregnancy rates
Holland Genetics Results: pregnancy rates Fresh IVP embryos: 45% - 55% Frozen embryos: IVP 40% - 50% SO 50% - 60%
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Results: Efficiency Oocytes: 8 9 Cleavage: 5,6 (70%)
OPU-IVP MOET Oocytes: 8 9 Cleavage: 5,6 (70%) Embryo: 1,6 (20%) 5 (55%) Calf: 0,8 (10%) 3 (33%)
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Insire embryo program
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Number of InSire-embryos per year
InSire-embryo donors Delta test heifers (IVP + SO) Delta donor cows (IVP + SO) Total from Delta program = 3000 Marker selected Bullmothers in West-Europe. (e.g. France, Germany, Belgium, Denmark and the Netherlands Totaal 4000 InSire-embryos
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Costs InSire-embryos InSire-embryos with option € 325,-
InSire-embryos with segment option € 275,- CRV claims /buy 15-20% of the calves born male € 1000 female € 3000
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Segmentation InSire-embryos
Kg Milk % Protein Life span en health Top exterior
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InSire-embryos offered by Internet
1) Random embryo selection (most actual combination) Choice of segment ( 4 ) Embryo price € 275 IVP fresh , IVP and SO frozen 2) Newest InSire embryos (most actual combination) Choice of combination Embryo price € 325 3) Available InSire embryos (in stock) IVP and SO frozen
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2) Newest InSire embryos (most actual combination)
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Benefits to the clients
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Win-Win situation Client InSire embryos: - Top class calves for a relative low price CRV: - increased genetics of bulls and bull dams against an acceptable costs
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Thank you for your attention!
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