1. Hematopoeisis
VIBS 443/602

2. Peripheral blood smear (May-Grunwald-Giemsa)
113
Peripheral blood smear (May-Grunwald-Giemsa)

3. 110
Peripheral blood smear (Leishman-Giemsa) eosinophil, basophil, and neutrophils

4. Bone marrow
19761

7. Bone marrow smear; low power view, showing negative images of fat globules

8. Megakaryocyte: contain many joined nuclei
Megakaryocyte: contain many joined nuclei. Each probably contain 8-32 times a normal diploid amount of DNA
Nuclei of megakaryocyte
Red blood cell

9. Macrophage: contains greenish, iron-containing granules due to poor quality. Differentiating cells have been torn away during preparation.
Macrophage
Lymphocyte
Neutrophil

10. Pronormoblast
Basophilic normoblast
Polychromatophilic normoblast
Orthochromatic normoblast
Myelocyte
Late metamyelocytes

11. Pronormoblast
Basophilic normoblast
Polychromatophilic normoblast
Orthochromatic normoblast
Myelocyte
Late metamyelocytes

12. Late promyelocyte
Late myelocyte/early metamyelocyte
Nearly mature PMN

13. Smudge cell
Promyelocyte
Myelocyte
Metamyelocytes

14. Pronormoblast
Metamyelocytes
Band cell

15. Bone marrow: EM 12a
Reticular cell
Developing red blood cell

16. Bone marrow; EM 8a
Lymphocyte
PNM
Red blood cell
eosinophil

17. Bone marrow; EM 12b
PMN
Immature neutrophil
Red blood cell

18. Routine H&E of bone
Marrow spicules
Lipid in fat cells

19. H&E of bone marrow
1. megakaryocytes

20. 19761

21. H&E of bone marrow:
Red blood cell

22. Rib
420

23. 420
Rib

24. 421
Tibia, fetal

25. 32583

26. 32583

27. Red bone marrow smear (May-Grunwald-Giemsa)
112
Red bone marrow smear (May-Grunwald-Giemsa)

30. Cells of Erythropoisesis
Pronormoblast
round nucleus and thin rim of deep blue cytoplasm
Basophilic normoblast
nuclear condensation ("fractured nucleus") with a deep blue cytoplasm

31. Polychromatophilic normoblast
Polychromatophilic normoblast
cytoplasm has accumulated enough hemoglobin so that it is no longer blue but a bluish-gray to bluish-red
Orthochromatic normoblast
must have an almost mature cytoplasm with only the faintest hint of blue: the nucleus is fully condensed

32. Reticulocyte
no nucleus, mature cytoplasm with blue tint
Red blood cell
typical of mature cell seen in circulation

35. Cells of Granulopoiesis
Promyelocyte
oval nucleus with deep blue cytoplasm and azurophilic granules
Myelocyte
cytoplasm becomes increasingly mature (reddish/clear) but still contains blue, nucleus of variable shape

36. Metamyelocyte
deeply indented nucleus with nearly mature cytoplasm (no longer blue)
Band cell
nucleus just prior to lobulation with fully mature cytoplasm
Neutrophil (mature)
lobulated nucleus with fully mature cytoplasm