1. USE OF OXYDOL TO TREAT SOIL CONTAMINATED WITH HYDROCARBONS. The Colombian Experience MARCELO ATEHORTUA HERRERA BIOPHARM S.A. VARICHEM DE COLOMBIA. BIOPHARM S.A. Edificio Century Tower Piso 18, Oficina 1818, Panamá. Tel.(507) 3947536 Fax. (507) 3947537 infocliente@biopharmpanama.com

2. OXYDOL, Treatment of soil contaminated with hydrocarbons. OXYDOL A product based on specific beneficial bacteria, enzymes, organic catalyzers, stabilizers and accelerator elements for the control of odors and to degrade organic matter in earth contaminated with hydrocarbons, restoring the soil for productive activities while complying with all applicable environmental regulations.

3. OXYDOL, Treatment of soil contaminated with hydrocarbons. OXYDOL Oxydol is a biologically safe alternative for environmental programs in areas of petroleum exploitation, whose application both with contaminated soil and liquid byproducts of hydrocarbon storage tanks (sediments) offers significant environmental benefits and improves the condition of soil allowing it to be used for other productive purposes

4. OXYDOL, Treatment of soil contaminated with hydrocarbons. GOAL Bioremediation of soil contaminated with hydrocarbons, reducing the rate of contamination to below the official minimum, 3000 ppm of TPH (Total Hydrocarbons Present), controlling unpleasant odors, returning the physiochemical condition of the soil to normal and promoting better environmental conditions for the area.

5. OXYDOL, Treatment of soil contaminated with hydrocarbons. PREPARATION To produce 20 liters of concentrated liquid OXYDOL, we have to mix 500 grams of powdered OXYDOL with 19.5 liters of water (free from chlorine).

6. OXYDOL, Preparation of the Product. Mix 500 grams of OXYDOL with19.5 liters of water Forming a concentrated aqueous solution of 20 liters of liquid OXYDOL.

7. OXYDOL, Description of the process Zero Subproducts Parameters of the Process Soil – Contaminated Residue Impermeabilized Biorremdiation Area Soil – Decontaminated Residue

8. OXYDOL, Applied Technology OXYDOL reproduces native microorganisms exponentially, achieving a greater quantity of microorganisms able to degrade hydrocarbons in less time. The excess population of native bacteria thus created is kept alive for the time required by the process by means of optimal conditions for them such as the oxygenation by ploughing, humidity, pH levels and several other parameters.

9. OXYDOL, Biorremediation Process Preparation of the land where the work is to be carried out. The area is examined and drainage, boundaries and marking are verified, as well as the need for impermeabilization, cleaning and levelling.. Intake, registration and listing of the afected material and subsequent placing and distribution within the area to be treated. Preparation of the product. Collecting of speciments to determinal initial levels of contamination of the soil, by means of the analysis of Total Hydrocarbons Present – TPH..

10. OXYDOL, Biorremediation Process Application of OXYDOL and encapsulation of oxygen necessary for degredation In order to conserve optimal treatment conditions, the ground will undergo a process of Mechanical Oxygenation, and a process of moistening, that depends on the climactic conditions of the area.

11. Soil after treatment is finalized, on the 27th day. OXYDOL, Biorremediation Process Taking samples to control and monitor the process.

12. OXYDOL, Results. PARAMETERSLABORATORYRESULTS VOLUME TREATED1200 M³ Bache 4 en LY 29 PRODUCT USEDOXYDOL RESULT – INITIAL SAMPLE. PRODYCOMTPH: 8369 mg/kg (ppm) ANTEKTPH: 6830 mg/kg (ppm) pHpH: 6.02 RESULT – FINAL SAMPLE. PRODYCOMTPH: 2800 mg/kg (ppm) ANTEKTPH: 2425 mg/kg (ppm)

13. OXYDOL, Results Reduction in the use of machinery Reuction in the use of additional products required by the Process Reduction in time taken by the process. Significant reduction of odors. BENEFITS Initial Sample, 8.369 ppm Volume treated, 1.200 m ³ Final Sample 2.800 ppm

14. OXYDOL, Treatment of soil contaminated with hydrocarbons. EVALUATIONS Analysis of TPH (Total Hydrocarbons Present). Design and follow-up of the protocol. Inspection and review of the physical condition of the soil and the environment as regards reduction of odors. Advice from personnel specialized in environmental matters.

15. OXYDOL, Treatment of soil contaminated with hydrocarbons. CONCLUSIONS During the process of biorremediation, the reduction of odors was remarkable after the first 15 days. The rapid degradation of hydrocarbons, was evident from laboratory analyses, results below TPH: 3000 mg/kg (ppm). The change in color of the soil and the change of its physicochemical properties was 90% completed on the 27th day of treatment. The physicochemical propertes of the soil comply with all requirements of the environmental authorities after the first month of treatment.

16. OXYDOL, Treatment of soil contaminated with hydrocarbons. CONCLUSIONS The inspection and design of appropriate protocols for environmental and geographical conditions and as regards the level of hydrocarbon contamination for the purpose of garantizing optimal results with this technology are definitive. The use of OXYDOL showed itself to be a rapid, safe and cost effective tool with many economic benefits for the briorremediation of soil contaminated with hydrocarbons, recovering the land and rendering it useful for other productive purposes.

17. OXYDOL Is produced by AGRANCO CORP U.S.A and Distributed in Colombia, Central America and the Caribbean by BIOPHARM S.A. O