1. Update on PED Research Lisa Becton, DVM, MS, DACVPM Dir. Swine Health Information & Research National Pork Board

2. Current Status of PED PED continues to be a major health challenge for U.S. producers into 2014 Since it was first diagnosed in May of 2013, there have been a total of 2962 cases reported: – CASE reports are different than SITE reports. – IA = 1095; OK = 290; NC = 367; MN = 396; KS = 171 – Data can be found at www.aasv.orgwww.aasv.org

3. PED case #’s/month (as of Jan 31 st, 2013)

4. National Pork Board Focus Research focus - New virus therefore needed research on PEDv and impact for US producers Development and communication of producer information/resources Containment/management strategies – Work with USDA/stakeholders and develop next steps for emerging disease response – Other needs?

5. PED Research Efforts

6. Development of Research Priorities *Swine Health Committee – producers, veterinarians, advisors (university, government, industry) PED Strategic Task Force Input from AASV and NPPC membership State Pork Association input

7. PEDv Research Priorities Initial Research Priorities:  Basic understanding of the characteristics and pathogenesis of PEDv and development of standard samples for use  Development and validation of diagnostic testing for antigen and antibody detection for PEDv and for oral fluids  Environmental stability of the virus on various surfaces and substrates and effectiveness of sanitation efforts  Epidemiology of the disease 7

8. Funded PEDv Research – June 2013 Hesse, KSU: 13-228 Tissue localization, shedding, virus carriage, antibody response and aerosol transmission of Porcine Epidemic Diarrhea virus (PEDV) following inoculation of feeder pigs Wang, OSU: 13-222 Propagation of PEDV in tissue culture and development of standardized reference samples for use in diagnostic testing Collins, UMN: 13-238 Development and validation of isolation and diagnostic testing detection for PEDv Collins, UMN: 13-239 Development and validation of diagnostic testing for antigen and antibody detection for PEDV Yoon, ISU: 13-226 Oral fluid testing for cost-effective and efficient surveillance and control of porcine epidemic diarrhea virus in swine populations

9. Funded PEDv Research (continued) Morrison, UMN: 13-216 Epidemiologic investigation on propensity for lateral spread of PED virus Goyal, UMN: 13-215 Environmental Stability of PEDV (Porcine Epidemic Diarrhea virus) Holtkamp, ISU: 13-227 Evaluation of time and temperature sufficient to kill PEDV in swine feces on metal surfaces Holtkamp, ISU: 13-248 Evaluation of the efficacy of Stalosan®F for inactivating porcine epidemic diarrhea virus (PEDv) and PRRS in swine feces on the surface of trailers used to haul live pigs

10. PEDv Research for Fall 2013 RFP was posted at www.pork.org/Researchwww.pork.org/Research Proposals submitted to NPB Sow immunity proposals selected by the Committee Remaining proposals that meet the call priorities will be reviewed Feb 19-20 th, 2014

11. Funded Research for Sow Immunity - 2013 Giménez-Lirola, ISU: 13-264 Defining PEDV maternal humoral immunity and correlates of neonatal protection Clement, SDSU: 13-263 Duration and level of PEDV neutralizing antibodies for clinical protection using feedback protocols Murtaugh, UMN: 13-262 PEDV feedback protocol optimization to improve immunity and productivity Since June 2013, the Board has approved >$1,000,000 for PED

12. Additional PED project funded 2013-2014 Pillatzki, ISU: 13-266 Feed transmission of PEDV to neonatal pigs Bowman, OSU: 14-150 Bioassay for Porcine Epidemic Diarrhea Virus contaminated feed Since June 2013, the Board has approved >$1,000,000 for PED Funding for 2014 - TBD

13. PEDv Research Focus for 2014 Committee discussion Feb 19-20 th on continuing needs for PED research, to include: – Further investigation of feed stuffs as vehicle for infection – Development of a rapid/pen-side test for feed – Assess capabilities for whole genome sequencing from current sample types – Cooperation, communication and collaboration are key to address this ongoing issue!

14. PED Research Information and updates on funded projects available at www.pork.org/PED www.pork.org/PED

15. This message funded by America’s Pork Checkoff Program. Thank you!lbecton@pork.org515-223-2791