1. G-Fectin TM 1. A novel transfection reagent developed by Genolution 2.Cationic lipid based 3. no cell splitting is required 4. Whole transfection can be done in 10 min 5. Your gene silencing in 24 hours 6. Lower toxicity than other reagents 7. Better gene silencing 8. For 500X transfection to one well of 24 well plate

2. G-Fectin has good Transfection efficacy Transfection assay using Vimentin siRNA final 10 nM

3. G-Fectin has low cytotoxicity Cell viability % All described cells were spitted in 30 % confluency in 24h ahead of viable cell counting with treatment of lipo-plexed RNA.

4. 10 min transfection protocol with G-Fectin with G-Fectin 50 ul of PBS 1-ul ofG-factin Genolution siRNA Add to 24 well plate directly Cells seed on plate Incubate 24 hrs qPCR Add the reagent & siRNA to the PBS Incubate 10 min at RT 450 ul of Suspended cells (5 x 10 4 cells/ well)

5. siRNA Transfection assay kit G-Fectin Kit TM – The easiest and fastest way for siRNA transfection assay test – Just add to the cell, wait for 48hours, and count the viable cell – The target gene of the used siRNA is essential for cell viability – The cell toxicity and level of the gene silencing is proportional Materials included scramble siRNA control siRNA (cause to cell death if transfected, for 200X assay ) siRNA dissolving buffer G-factin 0.5ml (for 500 X transfection to 24 well)

6. Easy siRNA Transfection assay kit Cell viability in 48 hour Scramble siRNA target siRNA Gene silencing in 24 hour

7. Materials included scramble siRNA control siRNA (cause to cell death if transfected ) siRNA dissolving buffer G-factin 0.5ml (for 500 X transfection to 24 well)