1. FSHN Safety Seminar August 27, 2008 Joe Przybyszewski Assistant Scientist FSHN Safety Officer Phone 294-5962 Office: 2583 FSB Email: jprzybys@iastate.edu

2. Outline  New Safety Information  Important Safety Reminders  FSHN game!

3. New Safety Information –Emergency alerts about severe weather, bomb threats or other immediate dangers will be sent through telephone call (to cell or landline), text message and e-mail. –Ensure that your contact information is New ISU Alert System! updated (in ACCESS PLUS, on your "Home" tab, click "ISU Alert" in the left navigation column). – For alternate severe weather & emergency email notification: KCCI.com (channel 8 news) emergencyemail.org Ames tornado, Nov 12, 2005 from: mesonet.agron.iastate.edu/

4. New Safety Information New Laboratory Safety Manual (LSM) is here! – Replaces Chemical Hygiene Plan. – Prefer to have the LSM on the lab computer (labeled by a sign) with internet access. – If no lab computer or internet access, then print out and place into LSM binder. – Important that you know what is in it, where it is and have access to it. – Change from being physically in the lab to being available electronically.

5. New Safety Information MUST BE IN THE LAB! – Material Safety Data Sheets (MSDS) – Standard Operating Procedures (SOP) – Emergency Action Plan Other Safety Documents – Still need a lab safety binder containing training records, chemical inventory & inspection documents. – Need to know where these are and have access to them.

6. Emergency Action Plan To be posted by all lab exits –Laboratory Emergency Action Plan Fire Medical emergency Intruder Vandalism Severe weather Utility Outages Spills Template available online from EHS –Link on FSHN website

7. New Safety Information Emergency Evacuation Maps are here! –Maps for each floor of each building are found on the EH&S website (under “Emergency Response”) –SOON will be posted in hallways and classrooms.

8. New Safety Information Please acquaint yourself with this safety information for your lab, office and classroom.

9. New Safety Information New “Chemicals of Interest (COI)” Inventory! – EH&S is responding to the Department of Homeland Security about Chemical Facility Antiterrorism Standards (CFATS). What do you need to do? – Keep your lab’s chemical inventory updated. – Review the list of COI (EH&S website under “lab safety”, then “chemical security”) and contact EH&S if you received new COI. http://pnt.gov/membership/dhs-large.png

10. New Safety Information New Biological Materials Inventory To meet the requirements of ISU’s policy and federal regulations, all researchers who use or store biological materials at ISU must submit a current inventory of biological materials to EH&S. To do this you may: A) enter biological materials into the EH&S database using the online biological inventory web form (must register as first- time user). OR B) submit a spreadsheet inventory and have it amended to the central database by using the upload link on the biological materials inventory page.

11. New Safety Information Biological Materials Inventory (continued) –Definition: Biological materials are any biologically derived material or any material which, either by accident or design, contains biological agents which might pose a risk to human or animal health or the environment. –Include: bacteria, fungi, plant pathogens, prions, biological toxins, parasites, viruses, recombinant DNA material, human blood, human cells and non-LAR transgenic or genetically-modified experimental animals, plants or insects.

12. New Safety Information Biological Materials Inventory (continued) Before you panic! Select whether the agent is a human/animal/insect/or plant pathogen, drug resistant or non-pathogenic. –If “non-pathogenic” is selected, no further info is needed. –If recombinant DNA is exempt from NIH guidelines (http://grants.nih.gov/grants/guide/notice-files/NOT-OD-02- 052.html), then no further info is needed. –If not, then, for example, the same corn (or bacterial) trait (or strain) can be combined into one entry if they have the same risk.

13. New Safety Information New Biosafety Manual – Defines biohazards, biosafety practices and procedures including disposal, disinfection and transportation of biohazardous materials. – Medical surveillance and biohazardous materials spill clean up.

14. New Safety Information –Nanotechnology is defined by the use of engineered particles with dimensions in the 1-100 nanometer (nm) range typically used as delivery devices. –Initial animal studies indicate that inhaled nanomaterials can cross the lung/blood barrier and deposit in internal organs. Skin penetration is another exposure route for nanoparticles. –Because nanomaterial toxicity is not fully understood, nanomaterials must be treated with a high level of control. Potential Concern - Nanotechnology! Nanoparticles above, buckyball; below, nanotube Both from Wikipedia

15. New Safety Information Nanotechnology Recommendations: –Register your use of nanomaterials by filling out a Nanomaterial User form (EH&S website under “Health and Safety”) & complete a Hazard Inventory form. –Treat nanomaterials as toxic substances. –EH&S must pick up nanomaterial waste. –See recommendations for safely working with nanomaterials at Iowa State University Nanomaterials Health and Safety Guidelines (EH&S website under “Health and Safety”).Iowa State University Nanomaterials Health and Safety Guidelines

16. New Safety Information –New Radiation Safety Manual! –All non-radiation users that work in a radiation lab (ie room with a Radionuclide Laboratory sign), must take “Radiation Safety for Non- Radiation Workers in a Radiation Laboratory” training! New Radiation Safety Information! – Also, “Radiation Safety for Non- Radiation Laboratory Employees” is recommended for all new graduate students who will be working in buildings containing radiation laboratories.

17. New Safety Information New from EH&S Website (http://www.ehs.iastate.edu/)

18. New Safety Information New from EH&S Website ( http://www.ehs.iastate.edu/) To help you and your supervisor determine which safety courses you need to take, perform a Needs Assessment Survey. under “Training”

19. New Safety Information New from EH&S Website ( http://www.ehs.iastate.edu/)

20. New Safety Information Free Chemicals!! – 10 pages of free chemicals. – free delivery New from EH&S Website

21. New Safety Information New online Hazardous Waste form – Replaces “Hazardous chemical waste acceptance form”. – Must still fill out the green waste tags.

22. New Safety Information Clean up your Lab/Office!! – how to get rid of unused chemicals, batteries, cleaning supplies, ink cartridges/toner and many other materials. New from EH&S

23. New Safety Information Updated FSHN Safety Website! http://www.fshn.hs.iastate.edu/faculty/safety.php

24. FSHN Safety Reminder – Safety training and completed quarterly and annual safety inspections are documented in the LSM or safety binder. – Templates for site-specific training document and check- in and check-out forms are on file in FSHN office (send to Joe Przybyszewski, 2312 FSB). – Update annual Chemical Inventory (send to Jim Gunning and Joe Przybyszewski, 2312 FSB). – Annual dept safety (and radiation) checklist completed by December and on file in FSHN Office (send to Joe Przybyszewski, 2312 FSB). – Update annual Biological Materials Inventory (performed online at EH&S website) FSHN Safety CheckList Requirements! (deadline 12/19/08) http://www.fshn.hs.iastate.edu/faculty/safety.php

25. FSHN Safety Reminders – No cloth on lab furniture! – E-waste Old unused copiers, printers, computers, monitors, etc. are considered E-waste and must be sent to Asset Recovery If these items were used in a lab, then a Laboratory Equipment Disposal Form (through EH&S) must be completed before sending to Asset Recovery. Because the backlog at Asset Recovery is usually 2-4 weeks, plan ahead. Don’t let e-waste sit out or you may be sited! Renovation Safety Reminders!

26. FSHN Safety Reminders –PIs need to coordinate moving of radioactive materials with EH&S ahead of time. –Freezer frost may be contaminated with radioactivity. Contact EH&S for proper surveys before defrosting a radiation-containing freezer. Radiation Safety Reminders!

27. FSHN Safety Reminders – 911 calls from campus phone goes right to ISU’s Department of Public Safety. – 911 calls from cell phone goes to Ames Story County Police. 911 Call Reminders!

28. FSHN Safety Reminders – Eyewash stations checked monthly – Container labeling – Chemical/biological inventories updated – Lab safety surveys performed annually Maximum fine is $27,500 per day per violation! Lab Survey Deficiencies!

29. FSHN Safety Reminders – Please make sure information is correct and dated. – Use permanent marker. – Post on outside of door. Emergency Contact Cards Date ________

30. FSHN Safety Reminders – Know the location of the emergency phone in your building in case you are locked out of your lab. – Do not work alone in the lab after hours or on weekends. Emergency Phones

31. Bomb Threats –If called, try to fill out the ISU Police checklist (link on FSHN safety website) –Forward email threats to ISU Police http://content.answers.com/main/content/wp/en/thumb/3/3b/300px-Classic_time_bomb.jpg http://www.zalmar.com/images/AEGIS-2510-ASH.jpg FSHN Safety Reminders

32. FSHN Safety News Pandemic Flu Planning! –Minimize risk to students, faculty & staff –Support students –Continue essential University operations –Resume normal operations as soon as possible 1918 Influenza Pandemic killed 50 million people

33. FSHN Safety Reminder Biological Waste –Label bags with temp sensitive tape –Write your contact info on tape –Autoclave open bag 40 minutes –Close and place in black bag after autoclaving –Place in dumpster –Monthly performance tests of autoclaves is strongly recommended Call EH&S to obtain an autoclave indicator J. Stewart 515-294-3926

34. Safety Check-in for Students, Staff, Faculty I have read the Laboratory Safety manual I have completed a Hazard Inventory I have received Site- Specific Safety Training I know yearly lab inspections are required I understand proper procedures for managing and disposal of chemical waste

35. I may need to find a buddy for work at night I know where MSDS’s are in my lab I know the number to call if injured I know safety glasses and a lab coat are required I understand that the safety of new research methods must be discussed Safety Check-in for Students, Staff, Faculty

36. I understand that I must be trained in proper procedures to use laboratory equipment I understand the lab notebook format required in my lab Safety Check-in for Students, Staff, Faculty

37. I understand that before I leave the lab I must complete a check-out involving: Arrange disposal of all chemical wastes with EH&S Proper storing and labeling of all research material left Preparation of a complete inventory of research material left Clean up of my personal workspace Updating my lab notebooks Inspection Safety Check-out for Students, Staff, Faculty

38. FSHN

39. Question 1 What are 5 items of safety equipment found in a laboratory that need regular inspection?

40. Answers to Question 1 1. First Aid Kit 2. Fire Extinguisher 3. Fume/Biosafety Hood 4. Eyewash 5. Spill kit

41. Question 2 What are 4 items of safety equipment that are found OUTSIDE of the laboratory?

42. Answers to Question 2 1. Safety Shower 2. Fire Alarm Pull Station 3. Fire Extinguisher 4. Emergency Phone

43. Question 3 What are the 7 items of safety information that the FSHN department says –should be kept in addition to your Laboratory Safety Manual (LSM) ?

44. Answers to Question 3 Standard Operating Procedures (SOPs) MSDS Laboratory Chemical Inventory Personnel safety training records Quarterly lab inspection sheets Yearly lab inspection sheets EH&S Lab inspection audits

45. Question 4 What are the top 8 things –you shouldn’t do with your mouth in a lab? http://dentalhealthonline.net/prevention_mouth_guards.html

46. Answers to Question 4 Mouth pipette Eat Drink Chew gum Apply chapstick or makeup Chew on pencils/pens Touch with gloved or unwashed hands Taste chemicals http://www.public.asu.edu/~iangould/pics/matt7.jpg What’s wrong here?

47. Question 5 What 4 pieces of information –should be put on labels of secondary chemical containers? ?

48. Answers to Question 5 Proper chemical or common name (in English) Signal words (Danger, Warning, Caution) or hazard (irritant, corrosive) Date (received, prepared or opened) Initials of owner/user

49. Question 6 What 7 safety items –should be worn in the laboratory? What’s wrong here?

50. Answers to Question 6 Safety glasses/goggles Lab Coat Gloves Fully enclosed shoes Hearing protection Long pants Apron/face shield

51. Question 7 What 10 pieces of safety equipment should be in good working order –In all ISU laboratories ? http://www.melbar.com.br/lab2.jpg

52. Answers to Question 7 Hood/Biosafety cabinet Safety shower Eyewash station Chemical Spill kit Biohazard spill kit Laboratory Safety Manual First aid kit Telephone with 911 stickers Fire extinguisher Fire alarm pull station

53. Question 8 What 8 hazards do –safety glasses, goggles and face shields protect your eyes from?

54. Answers to Question 8 Acids/Bases Organic Solvents Liquids under pressure Biological fluids Flying particles (Dust) Ultra Violet light Βeta radiation Other’s fingers http://www.lanl.gov/news/albums/bioscience/AnthraxUVplate.sized.jpg

55. Question 9 What should you do if there is a fire in your laboratory?

56. Answers to Question 9 Call 911 Pull fire alarm Evacuate all personnel Assist injured personnel Meet in prearranged area Use Fire extinguisher –ONLY if the fire is small –user must be trained –extinguisher lasts only 6-8 seconds http://www.nmsu.edu/safety/news/news-items/tt-chem-lab-fire2.htm

57. FSHN Fire 1985 223C MacKay

58. Question 10 What 3 pieces of equipment –must be considered when working with or storing flammable solvents? What’s wrong here?

59. Answers to Question 10 Flammable storage cabinet –For closed containers Fume hood –For active lab activities Refrigerator/freezer designed for flammable liquids –For cold storage http://www.ehs.iastate.edu/publications/factsheets/pec.pdf

60. FSHN Explosion 2001 Human Nutritional Sciences 2104

61. Question 11 What 5 liquid wastes –cannot be disposed of down the sink? http://www.chem.hope.edu/~polik/labtour/sinkarea.html

62. Answers to Question 11 Organic solvents Radioactive liquids Biohazardous waste Acid/base solutions Hazardous chemical solutions http://dbhs.wvusd.k12.ca.us/webdocs/Mole/Lab-MIC-DecantWithStirRod.jpg What’s wrong here?

63. Question 12 What are the top 5 things –You should not do when working in a fume hood or biosafety cabinet? http://upload.wikimedia.org/wikipedia/commons/thumb/0/02/Fume_hood.jpg/800px-Fume_hood.jpg

64. Answers to Question 12 Forget to turn on power Store solvents Block back vents Block airflow on front edge –or working surface Work with UV lights on http://images.google.com/imgres?imgurl=http://biology.queensu.ca/~hodsonp/Websitepi cs/Research/fumehood.jpg&imgrefurl=http://biology.queensu.ca/~hodsonp/research.htm &h=378&w=393&sz=49&hl=en&start=54&tbnid=g4N9ELM8HYpRJM:&tbnh=119&t bnw=124&prev=/images%3Fq%3Dfume%2Bhood%26start%3D40%26gbv%3D2%26n dsp%3D20%26svnum%3D10%26hl%3Den%26sa%3DN