1. 3. Prelab Isolation of Chlorophyll and Carotenoids from Spinach Exp. 17 BOSTON UNIVERSITY DRESDEN SCIENCE PROGRAM ORGANIC CHEMISTRY LABORATORY CAS CH 203, FALL 2014

2. Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach

3. Chloroplasts contain colored compounds (pigments) which fall into two categories chlorophylls and carotenoids

4. Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach Chlorophyll b: -CHO group instead of shaded methyl group Pheophytin a: identical to chlorophyll a except that Mg 2+ is replaced by two H + Pheophytin b: identical to chlorophyll b except that Mg 2+ is replaced by two H +

5. Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach

7.  -Carotene  -Carotene  -Carotene Different position of the double bond Xanthophylls are oxidized derivatives of carotenes. They contain hydroxyl groups and are more polar.

8. Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach Carotene is responsible for the orange colour of the carrots and many other fruits and vegetables. Xanthophylls are oxidized derivatives of carotenes. They contain hydroxyl groups and are more polar.

9. Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach

11. Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach Technique 12 – Column Chromatography

24. Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach Technique 14 – Thin-Layer Chromatography