1. About Science Prof OnlineScience Prof Online PowerPoint Resources Science Prof Online (SPO) is a free science education website that provides fully-developed Virtual Science Classrooms, science-related PowerPoints, articles and images. The site is designed to be a helpful resource for students, educators, and anyone interested in learning about science. The SPO Virtual Classrooms offer many educational resources, including practice test questions, review questions, lecture PowerPoints, video tutorials, sample assignments and course syllabi. New materials are continually being developed, so check back frequently, or follow us on Facebook (Science Prof Online) or Twitter (ScienceProfSPO) for updates. Many SPO PowerPoints are available in a variety of formats, such as fully editable PowerPoint files, as well as uneditable versions in smaller file sizes, such as PowerPoint Shows and Portable Document Format (.pdf), for ease of printing. Images used on this resource, and on the SPO website are, wherever possible, credited and linked to their source. Any words underlined and appearing in blue are links that can be clicked on for more information. PowerPoints must be viewed in slide show mode to use the hyperlinks directly. Several helpful links to fun and interactive learning tools are included throughout the PPT and on the Smart Links slide, near the end of each presentation. You must be in slide show mode to utilize hyperlinks and animations. This digital resource is licensed under Creative Commons Attribution-ShareAlike 3.0: http://creativecommons.org/licenses/by-sa/3.0/ Alicia Cepaitis, MS Chief Creative Nerd Science Prof Online Online Education Resources, LLC alicia@scienceprofonline.com From the Virtual Microbiology Classroom on ScienceProfOnline.comVirtual Microbiology Classroom ScienceProfOnline.com Image: Compound microscope objectives, T. Port Tami Port, MS Creator of Science Prof Online Chief Executive Nerd Science Prof Online Online Education Resources, LLC info@scienceprofonline.com

2. Laboratory Exercise 2b Media & Culture Examining Colony Morphology & Interpreting Specialized Media Images: Mannitol Salt, MacConkey;s & Arm plate, T. Port From the Virtual Microbiology Classroom on ScienceProfOnline.comVirtual Microbiology ClassroomScienceProfOnline.com

3. What am I going to learn from Lab Topic #2? Media & Culture You will practice microbial collection techniques. Define and use aseptic technique in microbial culture and media preparation. Use enriched, complex, selective & differential media to culture microbes.selective & differential media Describe colony morphology and its relationship to microbial identification. Interpret results of microbial growth on various culture media.culture media Understand the ubiquitous nature of microbes. Image: Chimp brain in a jar, Gaetan Lee Chimp brain in a jar From the Virtual Microbiology Classroom on ScienceProfOnline.comVirtual Microbiology ClassroomScienceProfOnline.com

4. Specialized Bacterial Growth Media: McConkey’s, Mannitol Salt & Blood Agar Last week you obtained samples of bacteria from the environment and from your body. You plated those samples on TSY and on specialized selective & differential media.selective & differential media McConkey’s = lighter, purplish-pink Mannitol Salt = orangish-pink Blood Agar = very dark red Image: McConkey’s, Mannitol Salt & Blood Agar specialized media, T. Port McConkey’sMannitol Salt Blood Agar From the Virtual Microbiology Classroom on ScienceProfOnline.comVirtual Microbiology ClassroomScienceProfOnline.com

5. MacConkey's MacConkey's (MAC) Q: Is MacConkey’s selective? Explain. Q: Is MacConkey’s differential? Explain. Image: McConkey’s growing Salmonella on the left, and E. coli on the right, T. Port McConkey’s From the Virtual Microbiology Classroom on ScienceProfOnline.comVirtual Microbiology ClassroomScienceProfOnline.com Watch VIDEO: How to Interpret MacConkey’s Agar (MAC)

6. M annitol SaltM annitol Salt (MSA) Q: Is Mannitol Salt selective? Explain. Q: Is Mannitol Salt differential? Explain. Images: Sterile Mannitol Salt Agar, Positive & negative differential reaction on Mannitol Salt Agar, T. PortMannitol Salt Agar From the Virtual Microbiology Classroom on ScienceProfOnline.comVirtual Microbiology ClassroomScienceProfOnline.com Watch VIDEO: How to Interpret Mannitol Salt Agar (MSA)

7. Blood agarBlood agar (BAP) Q: Is Blood agar selective? Explain. Q: Is Blood Agar differential? Explain. Images: Beta-hemolysis, Alpha-hemolysis and a sterile plate of Blood Agar, T. PortBlood Agar From the Virtual Microbiology Classroom on ScienceProfOnline.comVirtual Microbiology ClassroomScienceProfOnline.com Watch VIDEO: How to Interpret Blood Agar (BAP)

8. Images: Arm plate, TSY with sample from dish washer, MacConkey’s with variety of colonies, all by T. Port MacConkey’s Microbial Colony Morphology From the Virtual Microbiology Classroom on ScienceProfOnline.comVirtual Microbiology ClassroomScienceProfOnline.com

9. Microbial Colony Morphology

10. Record Results on Lab Report #2 Question #5: Describe results from touch plates and arm plate (TSY). a. What is the difference between touch plates 1, 2 and 3? b. Is this what you expected? Question #6: Record and interpret results from nasal swab plates (TSY and Mannitol Salt).Mannitol Salt a. Did any of the colonies turn the Mannitol Salt medium yellow? b. What would it mean if they did? Question #7: Record and interpret results from throat swab plate (Blood agar).Blood agar a. Do you see Alpha hemolysis? Beta hemolysis? Gamma hemolysis? b. Which hemolysis pattern is bad news? Question #8: Record and interpret results of environmental samples, for each quadrant 1 – 4 (TSY and MacConkey’s).MacConkey’s a. Are MacConkey colonies pink or colorless? b. What does it mean if they are pink? Images: Arm plate, Mannitol Salt, Blood agar & MacConkey’s, all by T. Port From the Virtual Microbiology Classroom on ScienceProfOnline.comVirtual Microbiology ClassroomScienceProfOnline.com

11. When you’re done with your environmental and clinical plates, put them in the discard bin at back of lab. From the Virtual Microbiology Classroom on ScienceProfOnline.comVirtual Microbiology ClassroomScienceProfOnline.com

12. Confused? Here are links to fun resources that further explain microbiology media & culture: Media & Culture Laboratory Main Page on the Virtual Microbiology Classroom of Science Prof Online. Science Prof Online “Germs”, music by Weird Al Yankovic. Video by RevLucio.Germs Normal Flora webpage, by Douglas F. Fix. Interactive page where you can select an area of the body and learn which normal flora typically colonize that location.Normal Flora How to Interpret: MacConkey’s (MAC), Mannitol Salt (MSA) and Blood Agar (BAP) videos from Science Prof Online.MacConkey’sMannitol SaltBlood Agar How to Pour Bacterial Growth Media into Petri Dishes, video from Science Prof Online.How to Pour Bacterial Growth Media into Petri Dishes Bacterial growth video and narration, YouTube, Dizzo95..Bacterial growth Microbial Growth & Metabolism Main Page on the Virtual Microbiology Classroom of Science Prof Online. Science Prof Online E. coli population growth time lapse video.E. coli population growth (You must be in PPT slideshow view to click on links.) From the Virtual Microbiology Classroom on ScienceProfOnline.comVirtual Microbiology ClassroomScienceProfOnline.com

13. Are microbes intimidating you? Do yourself a favor. Use the… Virtual Microbiology Classroom (VMC) ! The VMC is full of resources to help you succeed, including:VMC practice test questions review questions study guides and learning objectives You can access the VMC by going to the Science Prof Online website www.ScienceProfOnline.com www.ScienceProfOnline.com Images: Staph, Giant Microbes; Prokaryotic cell, Mariana RuizGiant Microbes; Prokaryotic cell