1. Chapter 3 Proteins

3. Purpose Mostly for structure: skin, muscle, fingernails, hair

5. What is the “R” group? Variable part of the amino acid Amino Acid Chart (handout)Amino Acid Chart

11. Forming/Breaking Peptide Bonds

19. 0

20. Type of Proteins 8. Receptor Proteins Intercellular communication Bind to specific proteins (like hormones) Receptor Proteins

23. Note the H-bonds (between -O of carbonyl and -H of amino): coiling and pleating

25. Tertiary Folding: disulfide bridges Disulfide bridges formed between cysteine amino acids (Look at cysteine’s structure)

26. Disulfide Bridges Formed when cysteine’s sulfur join You Tube Videos Disulfide Bonds

27. Use the Amino Acid Reference Sheet Select the type of tertiary interaction as (1) disulfide(2) ionic (3) H bonds(4) hydrophobic A.Leucine and valine B.Two cysteines C.Aspartic acid and lysine D. Serine and threonine

28. ANSWERS Select the type of tertiary interaction as (1) disulfide(2) ionic (3) H bonds(4) hydrophobic A.4 Leucine and valine B.1 Two cysteines C.2 Aspartic acid and lysine D. 3 Serine and threonine

30. Two Basic Shapes Fibrous Strands Like hair, nails,skin, muscle fibers Globular Blobs Like hemoglobin, insulin, hormones, antibodies

31. Quaternary: combination of other shapes collagen

32. Identify the level of protein structure 1. Primary2. Secondary 3. Tertiary 4. Quaternary A. Beta pleated sheet B. Order of amino acids in a protein C. A protein with two or more peptide chains D. The shape of a globular protein E. Disulfide bonds between R groups

33. Identify the level of protein structure 1. Primary2. Secondary 3. Tertiary 4. Quaternary ANSWERS 2 Beta pleated sheet 1 Order of amino acids in a protein 4 A protein with two or more peptide chains 3 The shape of a globular protein 3 Disulfide bonds between R groups

34. Video Stuff Protein Denaturation Video (McGraw-Hill) Interactive Animations: Protein FoldingInteractive Animations Protein Structures Animation No Sound,but great molecular models

35. Denaturation Disruption of secondary, tertiary and quaternary protein structure by: heat/organics (Break apart H bonds and disrupt hydrophobic attractions) acids/ bases(Break H bonds between polar R groups and ionic bonds) heavy metal ions (React with S-S bonds to form solids) agitation (Stretches chains until bonds break)

36. NOTE: Denaturation does not break the primary structure Denaturation of an egg with a strong acid videoDenaturation of an egg with a strong acid video

37. Protein Extra Credit OPP Can bring in up to 5 labels (no repeats) for 1 point each with a protein term circled: – protein – peptide – amino

38. Proteins are Active DO PROTEIN ACTIVITY