3. Series I Pump and Prep Pump

4. Prior to removing the head, rotate the flag on the top of the motor so the piston is fully retracted. Remove thumb nuts or Allen nuts using a 3/16” Allen wrench and slide off the SST head.

5. Remove inlet check valve holder with a 1/2” wrench and slide out SST check valve. Place SST check valve in new inlet holder noting the arrow on flow direction. Screw new inlet holder into the head and torque to 75 inch pounds.

6. Remove one Phillips head screw from aluminum or SST spacer

7. Hold pressure down on spacer while removing the second screw in order to keep from twisting and breaking the piston

8. Slide out the spring and then the ceramic or SST piston

9. Place small amount of grease on end of piston (May be SST or PEEK) and insert piston into the pump mechanism

10. Slide spring back over the piston

11. Reinstall the two Phillips head screws that secure the aluminum or SST spacer

12. Slide the head over the piston and insert the two 5/32” Allen nuts or thumb nuts Working from side to side, lightly snug the nuts. Once seated, if Allen head nuts, torque the nuts to 30 to 35 inch pounds.

13. Flow direction Outlet check valve and holder Inlet holder and check valve Flow Direction Note: arrows on check valves should be pointing up

15. Mechanism Piston carrier

16. Mechanism Cam Piston Carrier Shaft Piston Carrier roller bearing Front of pump

17. Mechanism Flag Electronic Flag Sensor

18. Self Flush Pump Heads Inlet “T” Prime/Purge Valve Prime/Purge Port Flow UHPLC Pump

19. Outlet Filter Assembly Check Valve Holders Single check valves torque to 130 in/lbs Flow UHPLC Pump

20. Outlet Filter Assembly UHPLC Pump

22. Pump Heads Flow UHPLC Pump Inlet Check Valve Holder Outlet Check Valve Holder

23. Seal Removal and Insertion Tool Seal Insertion End Seal Removal End Procedure for Removing and Installing new seal 5ml High Pressure Seal

24. Insert seal removal tool into head and remove SST or PEEK back up washer Seal Removal and Insertion Procedure

25. Insert seal removal tool into head and press to one side and gently pull to remove seal Seal Removal and Insertion Procedure

26. Clean seal port by inserting a corner of Scotch-Brite pad into hole and gently rotate. Rinse and use compressed air to remove any residue. Seal Removal and Insertion Procedure

27. Place new seal on opposite end of extraction tool with SST spring exposed Seal Removal and Insertion Procedure

28. Insert seal straight in making sure not to roll or pinch the seal’s edge Seal Removal and Insertion Procedure

30. Piston Replacement Retracted Manually rotate pulley until piston is in the retracted position. Having the piston in the retracted position decreases the chances the piston will break when removing the pump head. Not Retracted Front of Pump

31. Head Removal and Piston Replacement Remove two Allen nuts or thumb nuts Slide head off Remove Self-flush housingSlide washer spacer off

32. Head Removal and Piston Replacement Lift up piston from saddle UHPLC piston is black Clean piston with Scotch-Brite. Rinse and use compressed air to remove any residue. Assemble parts in reverse order. Torque nuts to 30 in/lbs or hand tighten thumb nuts Series II, III are clear or ceramic

34. Head and Self-flush

35. Pump Head and Self-flush Assembly UHPLC Pump

36. High Pressure Head Assembly Direction of flow Dual inlet Check valves InletOutlet Single outlet Check valve UHPLC Pump

37. High Pressure Check Valves Inlet of check valve has one hole Outlet of check valve has three holes UHPLC Pump

38. Inlet and Outlet Check Valves UHPLC Pump

39. Upper check valve holder Single Capsule torque to 120 in lbs Lower check valve holder Duel Capsule torque to 130 in lbs UHPLC Pump

40. Inlet and Outlet Ports on High Pressure Head Inlet port has larger (0.081) diameter hole Outlet port has smaller (0.046) diameter hole UHPLC Pump

42. Flow Self Flush sits behind the high pressure pump head

43. Self Flush outlet Flow Self Flush inlet UHPLC Pump

44. Self-flush Assembly Inlet Check Valve Outlet Check Valve Seal Direction of flow UHPLC Pump

45. Self-flush seal replacement Seal tool Clean seal port by inserting a corner of Scotch-Brite pad into hole and gently rotate. Rinse and use compressed air to remove any residue. UHPLC Pump

47. Seal Replacement Outlet If the backup washer is still in the seal cavity, insert the flanged end of the Teflon tool into the head and pull straight out. To remove the seal, reinsert the flanged end of the Teflon tool into the head, press to the side and pull straight out and remove the old seal. Gently clean the head with the Scotch-bright pad to remove any residue. Also, clean the piston with the pad to be sure the piston surface is clean. Do not use any other device other than the Teflon tool to remove and install the seal. Using a different device my scratch the head rendering it useless. To insert the new seal, position the seal on other end of the insertion tool so the stainless spring is positioned out and visible to the eye. Position the insertion tool so the seal will be pressed into the head without crimping, rolling or damaging the side of the seal. Press the seal into the head until it is firmly seated in the bottom of the seal port in the head. Reinstall the backup washer. Gently slide the head onto the pump.

49. PEEK Head Assembly Inlet Outlet Outlet Assembly 1/16” Seat Port (2 Check Valve) Inlet Assembly 1/8” Seat Port (2 Check Valves) 1/16” Outlet Port 1/8” Inlet Port Direction of flow Torque holders to 15 in/lbs

51. Prior to removing the head, rotate the flag on the top of the motor so the piston is fully retracted. Loosen two screws on the front of the head using 1/8” Allen wrench and slide head straight off Mighty Mini Seal Replacement

52. Insert flared end of Teflon removal tool into center of seal, press to one side and pull out.

53. Place new seal on opposite end of extraction tool with SST spring facing up.

54. Press seal straight in. Do not insert seal in at an angle.

55. Correct placement of seal PEEK spacer. Incorrect placement of spacer. Raised section is facing out. Raised section should be inserted inward to hold seal in place.

56. Mighty Mini Check Valve Replacement Direction of flow Note arrows on Check valves Inlet Outlet

57. Piston Replacement Remove two Phillips head screws

58. Piston Replacement Remove spring

59. Piston Replacement Remove Piston

60. Piston Replacement PEEK Spacer SST Washer Spring Piston

61. Piston Replacement PEEK Spacer SST Washer Spring Piston

62. Piston Replacement Slide spacer with SST washer over the piston and retighten two Phillips Head screws. The small weep hole should be located at the bottom. Slide PEEK head back on and working from side to side, retighten the screws that secure the head.

64. Old New

65. Old New

66. Old New

67. Old New

68. Old New

69. Old New

70. Old New

71. Old New

72. Old New

73. Old New

74. Old New 110 Volt AC 12 Volt DC

75. Old New

76. Old New Cooling indicator

78. Water 1 cps Milk 3 cps SAE 10 Motor Oil 85-140 cps SAE 20 Motor Oil 140-420 cps SAE 30 Motor Oil420-650 cps SAE 40 Motor Oil650-900 cps Castrol Oil, Vegetable Oil1,000 cps Karo Syrup 5,000 cps Honey 10,000 cps Chocolate Syrup25,000 cps Ketchup 50,000 cps Mustard70,000 cps What is Viscosity? The measure of the resistance of a fluid to flow. What is Centipoise (cps)? Water is the standard by which all fluids are measured. Water is 1 cps @ 70 Degrees F.

79. Ways to help pump higher viscosity fluids: Elevate the fluid Increase temperature of the fluid Pressurize vessel housing the fluid Replace inlet and outlet check valves with larger diameter check valves

81. Constant Flow: Typical pump configuration Most pumps are constant flow Pump delivers specified FLOW Does not take into account pressure If pressure readout – pulse dampener w/ transducer Max pressure limited to pressure of pulse dampener PEEK -5000 psi SST-6000 psi Constant Pressure: Typical pump configuration Pump delivers specified PRESSURE Does not take into account flow……sometimes? No pulse dampener, uses Transducer “T” assembly Some pumps can be set above 9000 psi

82. Pulse dampener w/ transducer (Constant Flow) Transducer “T” Assembly (Constant Pressure) PEEK Stainless Steel (SST)

83. Constant Pressure: Does not take into account flow……sometimes? Constant pressure pump can be set up to run constant flow. 1)Press “Mode” button until Ml/Min LED is lit 2)Set flow rate to desired rate 3)Press “Mode” button until Hi/Pr LED is lit 4)Set hi pressure to a number that you know it will not reach running at the desired flow rate 5)Press Start/Run The pump will run up to the set flow rate and stay there.