1. Valve Washes LEAP Technologies

2. VSWII vs Active Wash LEAP VSW II CTC Active Wash System Number of solvent 42 Pumping system Syringe pump Solenoid Pump Maximum Pressure 60 psi ~15 psi Able to wash loop YesNo

3. VSWII vs Active Wash LEAP VSW II CTC Active Wash System Syringe wash solvent To waste To Injector Flow rate range 0-15ml/min Fixed 4.2 ml/min Triggering Contact closure PwrOut 1 & 2 Programming PAL Via Standalone GUI / Analyst Driver macro/cycle

4. VSWII vs Active Wash LEAP VSW II CTC Active Wash System Usable standalone YesNo Variable flow rate YesNo No. of step programmable 9N/A Syringe wash as standard NoYes

5. VSWII vs Active Wash LEAP VSW II CTC Active Wash System Standalone module YesNo Requires custom cycles/macros NoNo Multiple units on one PAL YesNo

6. Active Wash Station

7. Active Wash In-Situ

9. Active Wash Features (1) The Active Wash Station consists of two self priming micro pumps mounted underneath the valve drive. The Active Wash Station consists of two self priming micro pumps mounted underneath the valve drive. A special needle guide assembly and tubing set including check valve. A special needle guide assembly and tubing set including check valve. The IN port of the pumps are connected to the wash solvent bottles of the Fast Wash Station. The IN port of the pumps are connected to the wash solvent bottles of the Fast Wash Station. The solenoids are powered using Pwr-Out1 and Pwr-Out2 (meaning that this can not be used with a Fast Wash Station). The solenoids are powered using Pwr-Out1 and Pwr-Out2 (meaning that this can not be used with a Fast Wash Station). The OUT ports are connected to the waste port of the injection valve. The OUT ports are connected to the waste port of the injection valve. The flow to waste is controlled by a non-return/ pressure relief valve in the waste line. The flow to waste is controlled by a non-return/ pressure relief valve in the waste line.

10. Active Wash Features (2) The Active Wash Station does not require special macros or cycles. The Active Wash Station does not require special macros or cycles. The Injector, Wash1, Wash2 and Waste are all trained to the same X, Y, Z points The Injector, Wash1, Wash2 and Waste are all trained to the same X, Y, Z points After sample loading and switching of the valve the pumps are activated with a fixed flow of approx. 70µl/sec. or 4.2ml/min. After sample loading and switching of the valve the pumps are activated with a fixed flow of approx. 70µl/sec. or 4.2ml/min. Cleaning solvent flows through the valve groove, the inside of the needle seal and is aspirated with the injection syringe. Cleaning solvent flows through the valve groove, the inside of the needle seal and is aspirated with the injection syringe. After the selected syringe cleaning strokes the injection syringe needle is lowered and the remaining sample between valve waste port and check valve is flushed. After the selected syringe cleaning strokes the injection syringe needle is lowered and the remaining sample between valve waste port and check valve is flushed.

11. Active Wash Features (3) As with the regular Fast Wash Station two different solvents can be used. As with the regular Fast Wash Station two different solvents can be used. Wetted parts of the new Active Wash Station are PTFE and Perfluor. Wetted parts of the new Active Wash Station are PTFE and Perfluor. The Active Wash Station setup enables entire sample flow path cleaning in backflush direction and rinses valve engravings, needle seal and syringe at the same time. The Active Wash Station setup enables entire sample flow path cleaning in backflush direction and rinses valve engravings, needle seal and syringe at the same time. It can be ordered optionally together with new instruments or as upgrade to existing HTS PAL / HTC PAL’s. It can be ordered optionally together with new instruments or as upgrade to existing HTS PAL / HTC PAL’s.

12. VSW II

13. Tee Piece Assembly Designed for positive pressure washing Designed for positive pressure washing Allows valve toggling Allows valve toggling Entire sample flow path can be washed Entire sample flow path can be washed Low maintenance seals (and easy to change) Low maintenance seals (and easy to change)

14. VSW II Features Program and forget Program and forget Triggers via contact closure Triggers via contact closure Choice of any of 4 solvent in up to 9 steps Choice of any of 4 solvent in up to 9 steps –For each step you can specify Flow rate Flow rate Volume Volume Can generate >60 psi Can generate >60 psi Can be used to clean the loop Can be used to clean the loop