1. Bycatch composition differs between northern sites and southern sites. At the northern sites, horseshoe crab were associated with: little and winter skate (Leucoraja spp.) summer flounder (Paralichthys dentatus) windowpane flounder (Scophthalmus aquosus). At the southern sites, horseshoe crab were predominately found with: clearnose skate (Raja eglanteria) knobbed whelk (Busycon carica) channel whelk (Busycotypus canaliculatus) spider crab (Libinia spp.). Identifying species that are caught as bycatch in the horseshoe crab trawl fishery is important. Many of the species listed above are not managed species and the impacts of catching and discarding individuals are unknown. Funding by: National Marine Fisheries Service Horseshoe crab Research and Monitoring NMFS Grant # NA05NMF4751135 Bycatch associated with the Horseshoe Crab (Limulus polyphemus) Trawl Survey Larissa Graham, Brian Murphy, David Hata ljgraham@vt.edu Department of Fisheries and Wildlife Sciences, Virginia Polytechnic Institute and State University Data Collection Sampling occurred from eastern Long Island, New York to the Delmarva Peninsula, Virginia (Figure 5). Sites were randomly-selected based on distance from shore (inshore/offshore) and bottom topography (trough/non- trough). A total of 156 sites were sampled for bycatch composition during the fall of 2005 (n = 73) and 2006 (n = 83). Sampling was conducted aboard a chartered commercial stern trawler, using a benthic trawl net. The net was modified to maximize the capture of horseshoe crabs. After towing the net for fifteen minutes, all taxa were emptied onto the deck, identified, counted, and weighed (Figure 6). Depth, bottom temperature and salinity were also recorded at each site. = Fall 2005 sites = Fall 2006 sites Bycatch: A fishery’s impact on non-target species It is estimated that among the 27 major fisheries in the USA, 1.06 million tons of fish are discarded for every 3.7 million tons landed 1. Of these major fisheries, shrimp and bottom trawl fisheries are responsible for about 72% of total bycatch, or non-targeted individuals, by gear type 1. Why the horseshoe crab fishery? Horseshoe crabs are harvested using bottom trawl nets (Figure 2). This gear has potential to catch many non- target organisms (Figure 3). Currently, few data have been collected on bycatch in the horseshoe crab trawl fishery, leaving ecosystem-level effects unknown. 1. Harrington, J. M., R. A. Myers, et al. (2005). "Wasted fishery resources: discarded by-catch in the USA." Fish and Fisheries 6(4): 350-361. 2. Crowder, L. B. and S. A. Murawski (1998). "Fisheries bycatch: implications for management." Fisheries Management 23(6): 8-17. 3. Davis, M. W. (2002). "Key principles for understanding fish bycatch discard mortality." Canadian Journal of Fisheries and Aquatic Sciences 59: 1834-1843. Data Analysis Descriptive analysis: For each tow, total number of individuals and biomass were calculated for each taxa. Preliminary Results 1.Identify and quantify taxa caught by horseshoe crab trawl gear within the survey area. 2.Describe species assemblages within the survey area, specifically identifying assemblages that include horseshoe crab. 3.Identify the biotic and/or abiotic factors that determine species assemblages (i.e. local abundance of horseshoe crab, bottom type, temperature, salinity). Horseshoe crab trawl gear has potential to catch many non-target species. The majority of the catch was composed of skates and rays (Figure 4). Many species were present in more than 50% of tows during both years. Figure 4. Percent of total biomass of both surveys combined. Multivariate statistical analyses: Cluster analysis was used to group sites. Relationships will be identified between biotic (species assemblages) and abiotic (bottom type, bottom temperature, salinity, or depth) factors. Project Objectives Many trawl fisheries require bycatch reduction devices (BRDs), which allow certain species to escape. However, before BRDs can be developed, managers must identify which species are being caught. Figure 2. A crew member sorts through a typical catch off Ocean City, Maryland. Figure 3. Bycatch in the horseshoe crab trawl fishery. Finfishes (6%) Skate and rays (55%) Inverts (4%) Horseshoe crab (33%) Sharks (2%) N A total distance of 193.7 km was sampled (88.7 km in 2005, 105 km in 2006). Eighty taxa were caught during the two surveys (60 taxa in 2005, 72 taxa in 2006), including 47 species of finfish, from 33 families. Figure 6. All bycatch was identified, measured, and weighed. Percent of total biomass Some bycatch are kept and sold at fish markets, but most are thrown back and often die as a result of stress or trauma 2,3 (Figure 1). These losses may affect total population size, age structure, and sex ratios, as well as the overall food web, habitat, and other species within the ecosystem 2. http://www.njscuba.net/artifacts/ship_fishing.html Figure 1. Catch that is not valuable to fishers is discarded. Summer flounderWindowpane flounderLittle/winter skate Clearnose skateChannel whelkSpider crab Figure 5. Sites trawled during 2005 and 2006 surveys.