1. Chapter 15 Enzyme Specificity and Regulation

2. 15.1 Specificity Is the Result of Molecular Recognition 15.1.1 Enzyme Specificity Structural specificity Stereospecificity

3. 15.1.2 Hypothesis of Enzyme Action the “lock and key” hypothesis the “induced fit” hypothesis

4. 15.1.3 Active Site of Enzyme The specific site on the enzyme where substrate binds and catalysis occurs is called the active site.

5. 15.2 Allosteric Regulation Feedback inhibition (or regulation) A B C D Z E1 E2 E3 En A B C D Y E Z E1 E2 E3 E4En E3* E4*En*

6. Sigmoidal curve

7. Allosteric activator Allosteric inhibitor CO 2 + Glu + ATP CTP N-carbamoylaspartate Asp ATCase Carbamoyl phosphate Activation Inhibition

8. 15.3 Reversible covalent modification

9. 15.4 Proteolytic Activation

10. 15.4.1 Pancreatic Proteases Trypsinogen Enteropeptidase Trypsin Chymotrypsinogen Proelastase Chymotrypsin Elastase

12. 15.4.2 Blood Clotting Cascade

13. 15.5 Regulation of Enzyme Synthesis and breakdown 15.6 Isozyme