1. MBV2010/BIO2140 Colloquium, March 6

2. RULES Multiple choice Only one correct answer 30-60 seconds to answer No textbook, computer, mobile phone, please There will be a prize for the group with the most correct answers.

3. Which of the following statements is wrong? A Uracil is a pyrimidine base B Virus genomes can have a size of more than 2 million bp C Cytosine is often methylated in DNA D Some bacteriophages are retroviruses 1 ?

4. Which of the following statements is wrong? 1

5. Which of the following systems repairs DNA mismatches? A RBCD B MutSLH C UvrABC D DNA glycosylases 2

6. Which of the following systems repairs DNA mismatches? 2

7. DNA glycosylases are involved in A Base excision repair B Nucleotide excision repair C Direct repair of damaged bases D Repair of double strand breaks 3

8. DNA glycosylases are involved in 3

9. Which of the following DNA polymerases synthesizes DNA in a template-independent manner? A DNA polymerase I B DNA polymerase III C DNA polymerase IV D DNA polymerase ß 4

10. Which of the following DNA polymerases synthesizes DNA in a template-independent manner? 4

11. Most histone tail modifications occur on A Histone H2A B Histone H2B C Histone H3 D Histone H4 5

12. Most histone tail modifications occur on 5

13. Pairing of homologous chromosomes in meiosis is mediated by A Cohesins B Condensins C Homologous recombination D Cyclins 6

14. Pairing of homologous chromosomes in meiosis is mediated by 6

15. TRF2 is a protein that binds to A Centromeres B Replication forks C Telomeres D Origins of replication 7

16. TRF2 is a protein that binds to 7

17. Which of the following proteins resolves Holliday junctions? A RuvA B RuvB C RecA D Rad51c 8

18. Which of the following proteins resolves Holliday junctions? 8 Rad51c Rad51c and other eukaryotic proteins function like RuvC in E. coli

19. Which protein initiates homologous recombination in meiosis? A Spo11 B Dmc1 C MRX D Rad51 9

20. Which protein initiates homologous recombination in meiosis? 9

21. Spo11 is a A serine recombinase B transposase C type II topoisomerase D type I topoisomerase 10

22. Spo11 is a 10

23. What proteins are shown in this figure? A Xis and IHF B Xis and integrase C Integrase and IHF D none of the above 11

24. What proteins are shown in this figure? 11 IHF

25. The molecular weight of histones is between 12 A 11 – 21 kD B 6 – 12 kD C 20 – 25 kD D 28 – 35 kD

26. The molecular weight of histones is between 12

27. What are the most abundant transposons in the human genome? 13 A PolyA retrotransposons B DNA transposons C Endogenous retroviruses D LTR retrotransposons

28. What are the most abundant transposons in the human genome? 13

29. Which proteins are shown in this figure? 14 A RuvA (1) and RuvB (2) B RuvA (2) and RuvB (1) C RuvA (1) and RuvC (2) D RuvB (1) and RuvC (2) 1 2

30. Which proteins are shown in this figure? 14 1 2

31. The yellow protein in this figure is 15 A a DNA topoisomerase B a DNA polymerase C a DNA helicase D a primase

32. The yellow protein in this figure is 15

33. Insertion (IS) sequences are 16 A LINE retrotransposons B DNA transposons C LTR retrotransposons D SINE retrotransposons

34. Insertion (IS) sequences are 16

35. Which genome contains Ty transposons? 17 A Human B Drosophila C Yeast D E. coli

36. Which genome contains Ty transposons? 17

37. All transposons 18 A contain terminal repeats B contain a reverse transcriptase gene C contain at least one gene D are flanked by target site duplications

38. All transposons 18